Psoralen-Containing Vinyl Monomer for Conjugation of Double-Helical DNA with Vinyl Polymers

Mizuo Maeda*, Chitoshi Nishimura, Daisuke Umeno, Makoto Takagi

*この研究の対応する著者

研究成果: Article査読

29 被引用数 (Scopus)

抄録

We have synthesized a vinyl monomer having a psoralen moiety, which can form a photoadduct with double-helical DNA. The monomer 1 was proved to have an ability to crosslink DNA double strands through a photochemical reaction when irradiated by UV light. The resulting DNA having vinyl groups was copolymerized with a comonomer such as acrylamide and N-isopropylacrylamide to give rise to a DNA—vinyl polymer conjugate. A conjugation based on covalent bondings was verified by using gel electrophoresis; the conjugation efficiency was found to be dependent upon concentration of the monomer which had been used in the antecedent photochemical reaction. This monomer will be a useful tool when anchoring double-helical DNA on polymeric materials for separating and sensing DNA-binding substances.

本文言語English
ページ(範囲)527-531
ページ数5
ジャーナルBioconjugate Chemistry
5
6
DOI
出版ステータスPublished - 1994 11 1
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 生体医工学
  • 薬理学
  • 薬科学
  • 有機化学

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