Purification and characterization of an extracellular β-glucosidase from the wood-grown fungus Xylaria regalis

Ding Ling Wei*, Kohtaro Kirimura, Shoji Usami, Tsung Hui Lin

*この研究の対応する著者

研究成果: Article査読

38 被引用数 (Scopus)

抄録

Xylaria regalis, a wood-grown ascomycete isolated in Taiwan, produces β-glucosidase (EC 3.2.1.21) extracellularly. The β-glucosidase was purified to homogeneity by ammonium sulfate precipitation, ion-exchange, and gel filtration chromatography. The molecular mass of the purified enzyme was estimated to be 85 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. With p-nitrophenyl β-D-glucopyranoside (PNPG) as the substrate at pH 5.0 and 50°C, the K(m) was 1.72 mM and V(max) was 326 μmol/min/mg. Optimal activity with PNPG as the substrate was at pH 5.0 and 50°C. The enzyme was stable at pH 5.0 at temperatures up to 50°C. The purified β-glucosidase was active against PNPG, cellobiose, sophorose, and gentiobiose, but did not hydrolyze lactose, sucrose, Avicel, and o- nitrophenyl β-D-galactopyranoside. The activity of β-glucosidase was stimulated by Ca2+, Mg2+, Mn2+, Cd2+ and β-mercaptoethanol, and inhibited by Ag2+, Hg2+, SDS, and p-chloromercuribenzoate (PCMB).

本文言語English
ページ(範囲)297-301
ページ数5
ジャーナルCurrent Microbiology
33
5
DOI
出版ステータスPublished - 1996 11月 1

ASJC Scopus subject areas

  • 微生物学
  • 応用微生物学とバイオテクノロジー

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