Purification and characterization of toad prolactin

Kazutoshi Yamamoto, Tetsuya Kobayashi, Sakaé Kikuyama*

*この研究の対応する著者

    研究成果: Article査読

    18 被引用数 (Scopus)

    抄録

    Prolactin (PRL) was purified from anterior pituitary glands of adult toad (Bufo japonicus formosus) by extraction of acetone-dried pituitary powder with acid acetone and chromatography on DEAE-cellulose and Sephadex G-100. Purification was monitored by radioimmunoassay developed for bullfrog PRL. Toad PRL thus obtained is as potent as bovine PRL in promoting collagen synthesis in the bullfrog tadpole tail fin. HPLC of the toad PRL on a reverse-phase column shows that the preparation behaves as a homogeneous protein. The hormone has a molecular weight of 23,000 as determined by SDS gel electrophoresis. The isoelectric point is 5.5 as determined by analytical electrofocusing. The amino acid composition closely resembles that of bullfrog PRL.

    本文言語English
    ページ(範囲)104-109
    ページ数6
    ジャーナルGeneral and Comparative Endocrinology
    63
    1
    DOI
    出版ステータスPublished - 1986

    ASJC Scopus subject areas

    • 内分泌学

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