Purification and localization of a calcium-binding protein in human spermatozoa

Masahisa Nakamura*, Hiromichi Ishikawa, Kouhei Matsuda, Teruaki Iwamoto, Seiji Furuya, Tomoyo Yamanobe, Masayuki Komukai, Kazutoshi Yamamoto, Akihiro Tamura, Shoichi Okinaga, Kiyoshi Arai

*この研究の対応する著者

研究成果: Article査読

3 被引用数 (Scopus)

抄録

The purpose of this study was to purify a calcium-binding protein (CalBP) from human spermatozoa of 226 men and to determine the localization of this protein in spermatozoa. The sperm cells were extracted with 0.6 MKCI, and the KCI extract was then subjected to gel filtration and high performance liquid chromatography. Two CalBPs of Mr = 38 kDa and 52 kDa were found by 45Ca2+-binding on blotted proteins. Only a 52 kDa CalBP (CalBP-52) was purified to a final yield of 0.007%. The isoelectric point of this protein was 5.1. The human CalBP-52 reacted with rabbit antiserum directed against rat 52 kDa CalBP. An immuno-cytochemical study showed that this protein was localized on the sperm head. It is postulated that this protein may have important functions related to Ca2+-transport into sperm cells.

本文言語English
ページ(範囲)500-505
ページ数6
ジャーナルHuman Reproduction
7
4
出版ステータスPublished - 1992 4
外部発表はい

ASJC Scopus subject areas

  • 統計学、確率および不確実性
  • 応用数学
  • 生理学(医学)
  • 生殖医学
  • 産婦人科学
  • リハビリテーション
  • 発生生物学
  • 生理学

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