Purification and properties of bullfrog prolactin

K. Yamamoto, S. Kikuyama

    研究成果: Article査読

    53 被引用数 (Scopus)

    抄録

    Prolactin was purified from bullfrog adenohypophyses by extraction of acetone-dried powder with acid acetone and chromatography on DEAE-cellulose and Sephadex G100. The bullfrog prolactin had a molecular weight of 23,000 as determined by SDS gel electrophoresis. The isoelectric point was 5.76 when determined by electrofocusing. The amino acid composition of the purified prolactin closely resembles that of mammalian prolactin. The prolactin was found to be 3.6 times as potent as bovine prolactin in promoting collagen synthesis in the tadpole tail fin.

    本文言語English
    ページ(範囲)59-64
    ページ数6
    ジャーナルEndocrinologia Japonica
    28
    1
    出版ステータスPublished - 1981

    ASJC Scopus subject areas

    • Endocrinology

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