Purification of cytoplasmic actin by affinity chromatography using the C-terminal half of gelsolin

Takashi Ohki, Chikanori Ohno, Kotaro Oyama, Sergey V. Mikhailenko, Shin'ichi Ishiwata

研究成果: Article

16 引用 (Scopus)

抜粋

A new rapid method of the cytoplasmic actin purification, not requiring the use of denaturants or high concentrations of salt, was developed, based on the affinity chromatography using the C-terminal half of gelsolin (G4-6), an actin filament severing and capping protein. When G4-6 expressed in Escherichia coli was added to the lysate of HeLa cells or insect cells infected with a baculovirus encoding the beta-actin gene, in the presence of Ca2+ and incubated overnight at 4 °C, actin and G4-6 were both detected in the supernatant. Following the addition of Ni-Sepharose beads to the mixture, only actin was eluted from the Ni-NTA column by a Ca2+-chelating solution. The functionality of the cytoplasmic actins thus purified was confirmed by measuring the rate of actin polymerization, the gliding velocity of actin filaments in an in vitro motility assay on myosin V-HMM, and the ability to activate the ATPase activity of myosin V-S1.

元の言語English
ページ(範囲)146-150
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
383
発行部数1
DOI
出版物ステータスPublished - 2009 5 22
外部発表Yes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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