Quantification of genetically modified soybean by quenching probe polymerase chain reaction

Hidenori Tani, Naohiro Noda, Kazutaka Yamada, Shinya Kurata, Satoshi Tsuneda, Akira Hirata, Takahiro Kanagawa*

*この研究の対応する著者

研究成果: Article査読

15 被引用数 (Scopus)

抄録

Quenching probe (QProbe) polymerase chain reaction (PCR) is a simple and cost-effective real-time PCR assay in comparison with other real-time PCR assays such as the TaqMan assay. We used QProbe-PCR to quantify genetically modified (GM) soybean (Roundup Ready soybean). We designed event-specific QProbes for Le1 (soy endogenous gene) and RRS (recombinant gene), and we quantified certified reference materials containing 0.1, 0.5, 1, 2, and 5% GM soybean. The TaqMan assay was also applied to the same samples, and the results were compared. The accuracy of QProbe-PCR was similar to that of TaqMan assay. When GM soybean content was 0.5% or more, the relative standard deviations of QProbe-PCR were less than 20%. QProbe-PCR is sensitive enough to monitor labeling systems and has acceptable levels of accuracy and precision.

本文言語English
ページ(範囲)2535-2540
ページ数6
ジャーナルJournal of Agricultural and Food Chemistry
53
7
DOI
出版ステータスPublished - 2005 4月 6

ASJC Scopus subject areas

  • 化学 (全般)
  • 農業および生物科学(全般)

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