Quantitative analysis of thrombopoietin receptors on human megakaryocytes

Tomoaki Kuwaki, Tetsuya Hagiwara, Chizuru Yuki, Ikuko Kodama, Takashi Kato, Hiroshi Miyazaki

研究成果: Article

17 引用 (Scopus)

抄録

Thrombopoietin (TPO), or c-MPL ligand, is the primary regulator of megakaryocyte and platelet production. TPO receptors expressed on human megakaryocytes derived from peripheral blood (PB) and cord blood (CB) progenitors cultured in the presence of TPO have now been analyzed quantitatively. Like those on human PB platelets, TPO receptors on the cultured megakaryocytes exhibited a molecular mass of approximately 80 kDa. Various characteristics of PB- and CB-derived megakaryocytes indicated that the former were more mature than the latter. Both PB- and CB-derived megakaryocytes expressed a single class of high-affinity TPO receptors, with 1933 ± 772 (n = 3) and 184 ± 48 (n = 4) sites per cell, respectively. These data indicate that the number of TPO receptors on human megakaryocytes increases with cell maturation.

元の言語English
ページ(範囲)46-50
ページ数5
ジャーナルFEBS Letters
427
発行部数1
DOI
出版物ステータスPublished - 1998 5 1
外部発表Yes

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Thrombopoietin Receptors
Megakaryocytes
Blood
Chemical analysis
Fetal Blood
Thrombopoietin
Platelets
Blood Platelets
Molecular mass
human MPL protein
Ligands

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

これを引用

Quantitative analysis of thrombopoietin receptors on human megakaryocytes. / Kuwaki, Tomoaki; Hagiwara, Tetsuya; Yuki, Chizuru; Kodama, Ikuko; Kato, Takashi; Miyazaki, Hiroshi.

:: FEBS Letters, 巻 427, 番号 1, 01.05.1998, p. 46-50.

研究成果: Article

Kuwaki, T, Hagiwara, T, Yuki, C, Kodama, I, Kato, T & Miyazaki, H 1998, 'Quantitative analysis of thrombopoietin receptors on human megakaryocytes', FEBS Letters, 巻. 427, 番号 1, pp. 46-50. https://doi.org/10.1016/S0014-5793(98)00391-3
Kuwaki, Tomoaki ; Hagiwara, Tetsuya ; Yuki, Chizuru ; Kodama, Ikuko ; Kato, Takashi ; Miyazaki, Hiroshi. / Quantitative analysis of thrombopoietin receptors on human megakaryocytes. :: FEBS Letters. 1998 ; 巻 427, 番号 1. pp. 46-50.
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AU - Kato, Takashi

AU - Miyazaki, Hiroshi

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