Quantitative analysis of thrombopoietin receptors on human megakaryocytes

Tomoaki Kuwaki, Tetsuya Hagiwara, Chizuru Yuki, Ikuko Kodama, Takashi Kato, Hiroshi Miyazaki

研究成果: Article査読

17 被引用数 (Scopus)

抄録

Thrombopoietin (TPO), or c-MPL ligand, is the primary regulator of megakaryocyte and platelet production. TPO receptors expressed on human megakaryocytes derived from peripheral blood (PB) and cord blood (CB) progenitors cultured in the presence of TPO have now been analyzed quantitatively. Like those on human PB platelets, TPO receptors on the cultured megakaryocytes exhibited a molecular mass of approximately 80 kDa. Various characteristics of PB- and CB-derived megakaryocytes indicated that the former were more mature than the latter. Both PB- and CB-derived megakaryocytes expressed a single class of high-affinity TPO receptors, with 1933 ± 772 (n = 3) and 184 ± 48 (n = 4) sites per cell, respectively. These data indicate that the number of TPO receptors on human megakaryocytes increases with cell maturation.

本文言語English
ページ(範囲)46-50
ページ数5
ジャーナルFEBS Letters
427
1
DOI
出版ステータスPublished - 1998 5 1
外部発表はい

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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