Quantitative detection of immunoreaction using magnetite nanoparticles and Raman scattering spectroscopy

Sho Hideshima, Masahiro Yanagisawa, Atsushi Arakaki, Tadashi Matsunaga, Tetsuya Osaka

研究成果: Article

1 引用 (Scopus)

抄録

We developed a new quantitative detection method for an immunoreaction combining magnetite (Fe3O4) nanoparticle labeling with confocal Raman spectrometry. Magnetite nanoparticles are transparent, Raman active, and easily can be controlled easily by magnets in bimolecular reactions. Human Chorionic Gonadtropin (hCG) was selected as a target molecule for this investigation. Sandwich immunoreaction was performed on a dot-patterned substrate using two anti-hCG antibodies, which were the antibody immobilized on the substrate and the biotinylated antibody. The immunoreaction was microscopically visualized by reacting streptavidin-modified magnetite nanoparticles with the biotinylated antibody on the substrate. The adsorbed magnetite nanoparticles on the dot pattern were detected by Raman intensity imaging. A good linear relation between the integrated Raman intensity spectra at 220 cm-1 and hCG concentration was gained. This method may be applied toward the detection of other target molecules in the fields of biotechnology and biomedicine.

元の言語English
ページ(範囲)142-146
ページ数5
ジャーナルe-Journal of Surface Science and Nanotechnology
6
DOI
出版物ステータスPublished - 2008 5 31

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Magnetite Nanoparticles
Magnetite nanoparticles
Raman Spectrum Analysis
antibodies
Antibodies
magnetite
Raman scattering
Spectroscopy
Raman spectra
nanoparticles
Substrates
scattering
Immobilized Antibodies
spectroscopy
Molecules
Streptavidin
Biotechnology
Labeling
Spectrometry
biotechnology

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Materials Science (miscellaneous)

これを引用

Quantitative detection of immunoreaction using magnetite nanoparticles and Raman scattering spectroscopy. / Hideshima, Sho; Yanagisawa, Masahiro; Arakaki, Atsushi; Matsunaga, Tadashi; Osaka, Tetsuya.

:: e-Journal of Surface Science and Nanotechnology, 巻 6, 31.05.2008, p. 142-146.

研究成果: Article

Hideshima, Sho ; Yanagisawa, Masahiro ; Arakaki, Atsushi ; Matsunaga, Tadashi ; Osaka, Tetsuya. / Quantitative detection of immunoreaction using magnetite nanoparticles and Raman scattering spectroscopy. :: e-Journal of Surface Science and Nanotechnology. 2008 ; 巻 6. pp. 142-146.
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AU - Yanagisawa, Masahiro

AU - Arakaki, Atsushi

AU - Matsunaga, Tadashi

AU - Osaka, Tetsuya

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N2 - We developed a new quantitative detection method for an immunoreaction combining magnetite (Fe3O4) nanoparticle labeling with confocal Raman spectrometry. Magnetite nanoparticles are transparent, Raman active, and easily can be controlled easily by magnets in bimolecular reactions. Human Chorionic Gonadtropin (hCG) was selected as a target molecule for this investigation. Sandwich immunoreaction was performed on a dot-patterned substrate using two anti-hCG antibodies, which were the antibody immobilized on the substrate and the biotinylated antibody. The immunoreaction was microscopically visualized by reacting streptavidin-modified magnetite nanoparticles with the biotinylated antibody on the substrate. The adsorbed magnetite nanoparticles on the dot pattern were detected by Raman intensity imaging. A good linear relation between the integrated Raman intensity spectra at 220 cm-1 and hCG concentration was gained. This method may be applied toward the detection of other target molecules in the fields of biotechnology and biomedicine.

AB - We developed a new quantitative detection method for an immunoreaction combining magnetite (Fe3O4) nanoparticle labeling with confocal Raman spectrometry. Magnetite nanoparticles are transparent, Raman active, and easily can be controlled easily by magnets in bimolecular reactions. Human Chorionic Gonadtropin (hCG) was selected as a target molecule for this investigation. Sandwich immunoreaction was performed on a dot-patterned substrate using two anti-hCG antibodies, which were the antibody immobilized on the substrate and the biotinylated antibody. The immunoreaction was microscopically visualized by reacting streptavidin-modified magnetite nanoparticles with the biotinylated antibody on the substrate. The adsorbed magnetite nanoparticles on the dot pattern were detected by Raman intensity imaging. A good linear relation between the integrated Raman intensity spectra at 220 cm-1 and hCG concentration was gained. This method may be applied toward the detection of other target molecules in the fields of biotechnology and biomedicine.

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KW - Iron oxide

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