Quantitative determination of branched-chain amino acids in human plasma using pressure-driven liquid chromatography with pillar array columns

Y. Song, K. Takatsuki, M. Isokawa, T. Sekiguchi, J. Mizuno, T. Funatsu, S. Shoji, M. Tsunoda

研究成果: Conference contribution

抄録

We have succeeded for the first time to quantify biological compounds in human plasma using pillar array columns. A fast and quantitative method for the determination of branched-chain amino acids (BCAAs) in human plasma using pressure-driven liquid chromatography on a chip was developed. BCAAs were firstly derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), and then analysis of NBD-BCAAs was optimized to be finished in 100 s. With the help of an internal standard, the concentrations of BCAAs in human plasma samples were quantitatively determined. The developed method should be a useful tool for fast diagnosis in clinical field.

本文言語English
ホスト出版物のタイトル17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
出版社Chemical and Biological Microsystems Society
ページ1794-1796
ページ数3
ISBN(印刷版)9781632666246
出版ステータスPublished - 2013 1 1
イベント17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
継続期間: 2013 10 272013 10 31

出版物シリーズ

名前17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
3

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period13/10/2713/10/31

ASJC Scopus subject areas

  • Bioengineering

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