Quantitative evaluation of a gold-nanoparticle labeling method for detecting target DNAs on DNA microarrays

Hyonchol Kim, Hiroyuki Takei, Kenji Yasuda*

*この研究の対応する著者

研究成果: Letter査読

18 被引用数 (Scopus)

抄録

The detection sensitivity obtained when combining electron-microscopy counting with gold-nanoparticle labeling of target nucleic acids complementary to probe DNA microarrays with the detection sensitivity obtained when using optical-microscopy measurement of conventional fluorescent labeling. Target DNAs were reacted with probe DNAs on DNA microarrays on which micro-columns had been etched so the target DNA could be agitated. The targets were reacted with the other probe DNA immobilized onto the surface of the nanoparticles. The labeled target DNAs were measured by using field-emission scanning electron microscopy (FE-SEM) imaging and counting the nanoparticles. The detection sensitivity of nanoparticle-labeling measurement was 1000 times that of fluorescent-labeling measurement, and the S/N ratio of nanoparticle-labeling measurement was 100 times that of fluorescent-labeling measurement. The results indicate that the nanoparticle-labeling method using FE-SEM counting is sensitive enough that it has a possibility for measuring targets expressed in a cell without amplification such as polymerase chain reaction (PCR).

本文言語English
ページ(範囲)6-10
ページ数5
ジャーナルSensors and Actuators, B: Chemical
144
1
DOI
出版ステータスPublished - 2010 1月 29
外部発表はい

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 器械工学
  • 凝縮系物理学
  • 表面、皮膜および薄膜
  • 金属および合金
  • 電子工学および電気工学
  • 材料化学

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