TY - JOUR
T1 - Quantitative evaluation of closed-loop-shaped cardiomyocyte network by using ring-shaped electrode
AU - Nomura, Fumimasa
AU - Kaneko, Tomoyuki
AU - Hamada, Tomoyo
AU - Hattori, Akihiro
AU - Yasuda, Kenji
PY - 2012/6
Y1 - 2012/6
N2 - Re-entry of excitation in the heart is one of the abnormal phenomena that causes lethal arrhythmia and is thought to be induced by the looped structure of the excitation conduction pathway. To evaluate the geometrical pattern dependence of electrophysiological results, we fabricated three models of cardiomyocyte networks and compared their beating frequencies (BFs), amplitudes of a depolarization peak, and field potential durations (FPDs). The set of different closed-loop-shaped network models from 3 to 8mm in length showed the same BFs, amplitudes, and FPDs independent of their loop lengths, whereas the BFs and FPDs of 60 μm small clusters, and the FPDs of the 2mm open-line-shaped network model were different from those of a closed-loop-shaped network model. These results indicate that the mm order larger size of clusters might create lower BFs, and the closed-loop-shaped model may generate longer FPDs. They also suggest the importance of spatial arrangement control of the cardoimyocyte community for reproducible measurement of electrophysiological properties of cardiomyocytes, especially control of the closedloop formation, which might change the waveforms of FPDs depending on the difference in the geometry and conduction pathway of the cell network.
AB - Re-entry of excitation in the heart is one of the abnormal phenomena that causes lethal arrhythmia and is thought to be induced by the looped structure of the excitation conduction pathway. To evaluate the geometrical pattern dependence of electrophysiological results, we fabricated three models of cardiomyocyte networks and compared their beating frequencies (BFs), amplitudes of a depolarization peak, and field potential durations (FPDs). The set of different closed-loop-shaped network models from 3 to 8mm in length showed the same BFs, amplitudes, and FPDs independent of their loop lengths, whereas the BFs and FPDs of 60 μm small clusters, and the FPDs of the 2mm open-line-shaped network model were different from those of a closed-loop-shaped network model. These results indicate that the mm order larger size of clusters might create lower BFs, and the closed-loop-shaped model may generate longer FPDs. They also suggest the importance of spatial arrangement control of the cardoimyocyte community for reproducible measurement of electrophysiological properties of cardiomyocytes, especially control of the closedloop formation, which might change the waveforms of FPDs depending on the difference in the geometry and conduction pathway of the cell network.
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U2 - 10.1143/JJAP.51.06FK06
DO - 10.1143/JJAP.51.06FK06
M3 - Article
AN - SCOPUS:84863330524
VL - 51
JO - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes
JF - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes
SN - 0021-4922
IS - 6 PART 2
M1 - 06FK06
ER -