Quantized velocities at low myosin densities in an in vitro motility

Taro Q.P. Uyeda*, Hans M. Warrick, Stephen J. Kron, James A. Spudich

*この研究の対応する著者

研究成果: Article査読

157 被引用数 (Scopus)

抄録

An in vitro motility assay has been developed in which single actin filaments move on one or a few heavy meromyosin (HMM) molecules. This movement is slower than when many HMM molecules are involved, in contrast to analogous experiments with microtubules and kinesin. Frequency analysis shows that sliding speeds distribute around integral multiples of a unitary velocity. This discreteness may be due to differences in the numbers of HMM molecules interacting with each actin filament where the unitary velocity reflects the activity of one HMM molecule. The value of the unitary velocity predicts a step size of 5-20 nm per ATP, which is consistent with the conventional swinging crossbridge model for myosin function.

本文言語English
ページ(範囲)307-311
ページ数5
ジャーナルNature
352
6333
DOI
出版ステータスPublished - 1991
外部発表はい

ASJC Scopus subject areas

  • 一般

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