Radial glial cell-specific ablation in the adult Zebrafish brain

Yuki Shimizu, Yoko Ito, Hideomi Tanaka, Toshio Ohshima*

*この研究の対応する著者

    研究成果: Article査読

    9 被引用数 (Scopus)

    抄録

    The zebrafish brain can continue to produce new neurons in widespread neurogenic brain regions throughout life. In contrast, neurogenesis in the adult mammalian brain is restricted to the subventricular zone (SVZ) and dentate gyrus (DG). In neurogenic regions in the adult brain, radial glial cells (RGCs) are considered to function as neural stem cells (NSCs). We generated a Tg(gfap:Gal4FF) transgenic zebrafish line, which enabled us to express specific genes in RGCs. To study the function of RGCs in neurogenesis in the adult zebrafish brain, we also generated a Tg(gfap: Gal4FF; UAS:nfsB-mcherry) transgenic zebrafish line, which allowed us to induce cell death exclusively within RGCs upon addition of metronidazole (Mtz) to the media. RGCs expressing nitroreductase were specifically ablated by the Mtz treatment, decreasing the number of proliferative RGCs. Using the Tg(gfap:Gal4FF; UAS:nfsB-mcherry) transgenic zebrafish line, we found that RGCs were specifically ablated in the adult zebrafish telencephalon. The Tg(gfap:Gal4FF) line could be useful to study the function of RGCs.

    本文言語English
    ページ(範囲)431-439
    ページ数9
    ジャーナルGenesis
    53
    7
    DOI
    出版ステータスPublished - 2015 7月 1

    ASJC Scopus subject areas

    • 内分泌学
    • 細胞生物学
    • 遺伝学

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