Raman imaging of the diverse states of the filamentous cyanobacteria

J. Ishihara, M. Tachikawa, A. Mochizuki, Y. Sako, H. Iwasaki, S. Morita

研究成果: Conference contribution

1 被引用数 (Scopus)

抄録

The objective of our research was to predict cell fates of a multicellular system, accompanied by cellular differentiation. To fulfill this objective, we sought to distinguish the differentiated and undifferentiated cells of filamentous cyanobacteria (Anabaena sp. PCC 7120) using Raman imaging. This technique indicated Raman bands of the cellular system, in which several bands were assigned to vibrations of β-carotene and scytonemin. We applied principal component analysis (PCA) to the Raman spectra to determine the PC1 and PC2 loading plots and their scores. The data points obtained for heterocyst tended to converge along the bottom of the scatterplot whereas those for vegetative cells were more widely distributed in the PC plane. This indicates that the chemical compositions of a heterocyst were relatively stable. As vegetative cells are capable of proliferation or differentiation, they may transit and exist in several states including the pseudo-differentiated state. The results suggest that the chemical compositions of a vegetative cell fluctuated according to its cellular condition. In conclusion, the results of Raman imaging indicate that the diverse states of vegetative cells are localized in a specific state through differentiation.

本文言語English
ホスト出版物のタイトルNano-Bio Sensing, Imaging, and Spectroscopy
DOI
出版ステータスPublished - 2013 9月 17
イベントSPIE Nano-Bio Sensing, Imaging, and Spectroscopy - Jeju, Korea, Republic of
継続期間: 2013 2月 202013 2月 23

出版物シリーズ

名前Proceedings of SPIE - The International Society for Optical Engineering
8879
ISSN(印刷版)0277-786X
ISSN(電子版)1996-756X

Conference

ConferenceSPIE Nano-Bio Sensing, Imaging, and Spectroscopy
国/地域Korea, Republic of
CityJeju
Period13/2/2013/2/23

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 凝縮系物理学
  • コンピュータ サイエンスの応用
  • 応用数学
  • 電子工学および電気工学

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