Rapid Diversification of BetI-Based Transcriptional Switches for the Control of Biosynthetic Pathways and Genetic Circuits

Kazuya Saeki, Masahiro Tominaga, Shigeko Kawai-Noma, Kyoichi Saito, Daisuke Umeno*

*この研究の対応する著者

研究成果: Article査読

13 被引用数 (Scopus)

抄録

Synthetic biologists are in need of genetic switches, or inducible sensor/promoter systems, that can be reliably integrated in multiple contexts. Using a liquid-based selection method, we systematically engineered the choline-inducible transcription factor BetI, yielding various choline-inducible and choline-repressive promoter systems with various input-output characteristics. In addition to having high stringency and a high maximum induction level, they underwent a graded and single-peaked response to choline. Taking advantage of these features, we demonstrated the utility of these systems for controlling the carotenoid biosynthetic pathway and for constructing two-input logic gates. Additionally, we demonstrated the rapidity, throughput, robustness, and cost-effectiveness of our selection method, which facilitates the conversion of natural genetic controlling systems into systems that are designed for various synthetic biology applications.

本文言語English
ページ(範囲)1201-1210
ページ数10
ジャーナルACS Synthetic Biology
5
11
DOI
出版ステータスPublished - 2016 11月 18
外部発表はい

ASJC Scopus subject areas

  • 生体医工学
  • 生化学、遺伝学、分子生物学(その他)

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