Rapid DNA chemical ligation for amplification of RNA and DNA signal

Hiroshi Abe, Yuko Kondo, Hiroshi Jinmei, Naoko Abe, Kazuhiro Furukawa, Atsushi Uchiyama, Satoshi Tsuneda, Kyoko Aikawa, Isamu Matsumoto, Yoshihiro Ito

研究成果: Article

22 引用 (Scopus)

抜粋

Enzymatic ligation methods are useful in the diagnostic detection of DNA sequences. Here, we describe the investigation of nonenzymatic phosphorothioate-iodoacetyl DNA chemical ligation as a method for the detection and identification of RNA and DNA. The specificity of ligation on the DNA target is shown to allow the discrimination of a single point mutation with a drop in the ligation yield of up to 16.1-fold. Although enzymatic ligation has very low activity for RNA targets, this reaction is very efficient for RNA targets. The speed of the chemical ligation with an RNA target achieves a 70% yield in 5 s, which is equal to or better than that of ligase-enzyme-mediated ligation with a DNA target. The reaction also exhibits a significant level of signal amplification under thermal cycling in periods as short as 100-120 min, with the RNA or DNA target acting in a catalytic way to ligate multiple pairs of probes.

元の言語English
ページ(範囲)327-333
ページ数7
ジャーナルBioconjugate Chemistry
19
発行部数1
DOI
出版物ステータスPublished - 2008 1 1

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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  • これを引用

    Abe, H., Kondo, Y., Jinmei, H., Abe, N., Furukawa, K., Uchiyama, A., Tsuneda, S., Aikawa, K., Matsumoto, I., & Ito, Y. (2008). Rapid DNA chemical ligation for amplification of RNA and DNA signal. Bioconjugate Chemistry, 19(1), 327-333. https://doi.org/10.1021/bc700244s