Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method

N. Noda*, H. Ikuta, Y. Ebie, A. Hirata, S. Tsuneda, M. Matsumura, T. Sumino, Y. Inamori

*この研究の対応する著者

研究成果査読

3 被引用数 (Scopus)

抄録

Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed bacterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO 14298) and sixteen against Nitrobacter winogradskyi (IFO 14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and N. winogradskyi in a biofilm could be examined by in situ fluorescent antibody technique. It was shown that most of N. winogradskyi existed near the surface part and most of N. europaea existed at the inner part of the polyethylene glycol (PEG) gel pellet, which had entrapped activated sludge and used in a landfill leachate treatment reactor. It was suggested that this monoclonal antibody method was utilized for estimating and controlling the population of nitrifying bacteria as a quick and favorable tool.

本文言語English
ページ(範囲)301-308
ページ数8
ジャーナルWater Science and Technology
41
4-5
DOI
出版ステータスPublished - 2000
イベントBiofilms Systems IV - New York, NY, USA
継続期間: 1999 10 171999 10 20

ASJC Scopus subject areas

  • 環境工学
  • 水の科学と技術

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