Ras recruits mitotic exit regulator Lte1 to the bud cortex in budding yeast

Satoshi Yoshida, Ryuji Ichihashi, Akio Toh-e

研究成果: Article査読

51 被引用数 (Scopus)

抄録

ACdc25 family protein Lte1 (low temperature essential) is essential for mitotic exit at a lowered temperature and has been presumed to be a guanine nucleotide exchange factor (GEF) for a small GTPase Tem1, which is a key regulator of mitotic exit. We found that Lte1 physically associates with Ras2-GTP both in vivo and in vitro and that the Cdc25 homology domain (CHD) of Lte1 is essential for the interaction with Ras2. Furthermore, we found that the proper localization of Lte1 to the bud cortex is dependent on active Ras and that the overexpression of a derivative of Lte1 without the CHD suppresses defects in mitotic exit of a Δlte1 mutant and a Δras1 Δras2 mutant. These results suggest that Lte1 is a downstream effector protein of Ras in mitotic exit and that the Ras GEF domain of Lte1 is not essential for mitotic exit but required for its localization.

本文言語English
ページ(範囲)889-897
ページ数9
ジャーナルJournal of Cell Biology
161
5
DOI
出版ステータスPublished - 2003 6 9
外部発表はい

ASJC Scopus subject areas

  • 細胞生物学

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