Real-time detection of DNA hybridization on microarray using a CCD-based imaging system equipped with a rotated microlens array disk

Takeyuki Mogi, Keiichi Hatakeyama, Tomoyuki Taguchi, Hitoshi Wake, Takeo Tanaami, Masahito Hosokawa, Tsuyoshi Tanaka, Tadashi Matsunaga

研究成果: Article

13 引用 (Scopus)

抄録

This work describes a novel charge-coupled device (CCD)-based imaging system (MB Biochip Reader™) for real-time detection of DNA hybridization to DNA microarrays. The MB Biochip Reader™ consisted of a laser light source (532. nm), a microlens array for generation of a multi-beam laser, and a CCD for 2-D signal imaging. The MB Biochip Reader™ with a rotated microlens array, allowed large-field imaging (6.2. mm×7.6. mm with 6.45μm resolution) with fast time-resolution at 0.2. s without speckle noise. Furthermore, real-time detection of DNA hybridization, which is sufficient to obtain accurate data from tens of thousands of array element per field, was successfully performed without the need for laser scanning. The performance of the MB Biochip Reader™ for DNA microarray imaging was similar to the commercially available photomultiplier tube (PMT)-based microarray scanner, ScanArray Lite. The system potentially could be applied toward real-time analysis in many other fluorescent techniques in addition to real-time DNA microarray analysis.

元の言語English
ページ（範囲）1942-1946
ページ数5
ジャーナルBiosensors and Bioelectronics
巻26
発行部数5
DOI
出版物ステータスPublished - 2011 1 15
外部発表Yes

Fingerprint

Biochips
Microarrays
Charge coupled devices
Imaging systems
DNA
Equipment and Supplies
Oligonucleotide Array Sequence Analysis
Lasers
Imaging techniques
Photomultipliers
Microarray Analysis
Speckle
Laser beams
Light sources
Scanning
Light

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

これを引用

Real-time detection of DNA hybridization on microarray using a CCD-based imaging system equipped with a rotated microlens array disk. / Mogi, Takeyuki; Hatakeyama, Keiichi; Taguchi, Tomoyuki; Wake, Hitoshi; Tanaami, Takeo; Hosokawa, Masahito; Tanaka, Tsuyoshi; Matsunaga, Tadashi.

：: Biosensors and Bioelectronics, 巻 26, 番号 5, 15.01.2011, p. 1942-1946.

研究成果: Article

Mogi, T, Hatakeyama, K, Taguchi, T, Wake, H, Tanaami, T, Hosokawa, M, Tanaka, T & Matsunaga, T 2011, 'Real-time detection of DNA hybridization on microarray using a CCD-based imaging system equipped with a rotated microlens array disk', Biosensors and Bioelectronics, 巻. 26, 番号 5, pp. 1942-1946. https://doi.org/10.1016/j.bios.2010.07.015
Mogi T, Hatakeyama K, Taguchi T, Wake H, Tanaami T, Hosokawa M その他. Real-time detection of DNA hybridization on microarray using a CCD-based imaging system equipped with a rotated microlens array disk. Biosensors and Bioelectronics. 2011 1 15;26(5):1942-1946. https://doi.org/10.1016/j.bios.2010.07.015
Mogi, Takeyuki ; Hatakeyama, Keiichi ; Taguchi, Tomoyuki ; Wake, Hitoshi ; Tanaami, Takeo ; Hosokawa, Masahito ; Tanaka, Tsuyoshi ; Matsunaga, Tadashi. / Real-time detection of DNA hybridization on microarray using a CCD-based imaging system equipped with a rotated microlens array disk. ：: Biosensors and Bioelectronics. 2011 ; 巻 26, 番号 5. pp. 1942-1946.
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