抄録
This work describes a novel charge-coupled device (CCD)-based imaging system (MB Biochip Reader™) for real-time detection of DNA hybridization to DNA microarrays. The MB Biochip Reader™ consisted of a laser light source (532. nm), a microlens array for generation of a multi-beam laser, and a CCD for 2-D signal imaging. The MB Biochip Reader™ with a rotated microlens array, allowed large-field imaging (6.2. mm×7.6. mm with 6.45μm resolution) with fast time-resolution at 0.2. s without speckle noise. Furthermore, real-time detection of DNA hybridization, which is sufficient to obtain accurate data from tens of thousands of array element per field, was successfully performed without the need for laser scanning. The performance of the MB Biochip Reader™ for DNA microarray imaging was similar to the commercially available photomultiplier tube (PMT)-based microarray scanner, ScanArray Lite. The system potentially could be applied toward real-time analysis in many other fluorescent techniques in addition to real-time DNA microarray analysis.
本文言語 | English |
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ページ(範囲) | 1942-1946 |
ページ数 | 5 |
ジャーナル | Biosensors and Bioelectronics |
巻 | 26 |
号 | 5 |
DOI | |
出版ステータス | Published - 2011 1月 15 |
外部発表 | はい |
ASJC Scopus subject areas
- 生物理学
- 生体医工学
- バイオテクノロジー
- 電気化学