Real time imaging of single fluorophores on moving actin with an epifluorescence microscope

I. Sase, H. Miyata, J. E T Corrie, J. S. Craik, K. Kinosita*

*この研究の対応する著者

研究成果: Article査読

98 被引用数 (Scopus)

抄録

Relatively simple modifications of an ordinary epifluorescence microscope have greatly reduced its background luminescence, allowing continuous and real time imaging of single fluorophores in an aqueous medium. Main modifications were changing the excitation light path and setting an aperture stop so that stray light does not scatter inside the microscope. A simple and accurate method using actin filaments is presented to establish the singularity of the observed fluorophores. It was possible, at the video rate of 30 frames/s, to image individual tetramethylrhodamine fluorophores bound to actin filaments sliding over heavy meromyosin. The successful imaging of moving fluorophores demonstrates that conventional microscopes may become a routine tool for studying dynamic interactions among individual biomolecules in physiological environments.

本文言語English
ページ(範囲)323-328
ページ数6
ジャーナルBiophysical Journal
69
2
出版ステータスPublished - 1995
外部発表はい

ASJC Scopus subject areas

  • 生物理学

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