@inproceedings{eb01fe7ace864ead9505f902fa0cf3f3,
title = "Real-time secretion analysis revealed correlation of IL-1β release and loss of cell membrane integrity",
abstract = "Live-cell imaging of inflammatory cytokine secretion from human monocytes was successfully performed on a microfabricated well array chip by time-resolved fluoroimmunoassay using total internal reflection fluorescence microscopy. The system enabled to monitor secretion activity from tens of cells at a time resolution of 1 min. We first observed the close correlation between the loss of cell membrane integrity and {"}non-classical secretion{"} of IL-1β cytokine of inflammasome activated human peripheral monocytes.",
keywords = "Real-time secretion analysis, Single cell, TIRF",
author = "Y. Shirasaki and M. Yamagishi and K. Izawa and K. Nakagawa and A. Nakahara and N. Suzuki and J. Mizuno and T. Sekiguchi and T. Heike and R. Nishikomori and S. Shoji and O. Ohara",
year = "2013",
month = jan,
day = "1",
language = "English",
isbn = "9781632666246",
series = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
publisher = "Chemical and Biological Microsystems Society",
pages = "1686--1688",
booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
note = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 ; Conference date: 27-10-2013 Through 31-10-2013",
}