Real-time secretion analysis revealed correlation of IL-1β release and loss of cell membrane integrity

Y. Shirasaki*, M. Yamagishi, K. Izawa, K. Nakagawa, A. Nakahara, N. Suzuki, J. Mizuno, T. Sekiguchi, T. Heike, R. Nishikomori, S. Shoji, O. Ohara

*この研究の対応する著者

研究成果: Conference contribution

抄録

Live-cell imaging of inflammatory cytokine secretion from human monocytes was successfully performed on a microfabricated well array chip by time-resolved fluoroimmunoassay using total internal reflection fluorescence microscopy. The system enabled to monitor secretion activity from tens of cells at a time resolution of 1 min. We first observed the close correlation between the loss of cell membrane integrity and "non-classical secretion" of IL-1β cytokine of inflammasome activated human peripheral monocytes.

本文言語English
ホスト出版物のタイトル17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
出版社Chemical and Biological Microsystems Society
ページ1686-1688
ページ数3
ISBN(印刷版)9781632666246
出版ステータスPublished - 2013 1 1
イベント17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
継続期間: 2013 10 272013 10 31

出版物シリーズ

名前17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
3

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
国/地域Germany
CityFreiburg
Period13/10/2713/10/31

ASJC Scopus subject areas

  • バイオエンジニアリング

フィンガープリント

「Real-time secretion analysis revealed correlation of IL-1β release and loss of cell membrane integrity」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル