Recognition of tRNA bottom half by bacterial ribonuclease P.

Yasuhiro Nagai, Tomoaki Ando, Terumichi Tanaka, Yo Kikuchi

研究成果: Chapter

抄録

Bacterial ribonuclease P (RNase P) contains a catalytic RNA that cleaves precursor tRNA to form the 5'-end of mature tRNA. Bacterial RNase P mainly recognizes the acceptor stem and T arm modules of tRNA molecules. The region consisting of T arm, acceptor stem and 3' CCA motif in the tRNA is generally termed "top half", and the region consisting of the others, anticodon arm, extra loop and D arm, is called "bottom half". The stems in the top half contribute to recognition, but effects of the bottom half have not been elucidated. To study the effects of the bottom half on the RNase P recognition, we have synthesized several mutant substrates that have the bottom half on different positions along the top half stem. Most of these mutants were cleaved by Escherichia coli RNase P precisely at the expected position, but the cleavage efficiencies were very different especially at low Mg2+ concentration. We also found that RNase P holoenzyme prefered somewhat mutated tRNA precursor to the wild-type tRNA precursor.

元の言語English
ホスト出版物のタイトルNucleic acids research. Supplement (2001)
ページ281-282
ページ数2
エディション3
出版物ステータスPublished - 2003
外部発表Yes

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Ribonuclease P
Transfer RNA
RNA Precursors
Anticodon
Catalytic RNA
Holoenzymes
Escherichia coli

これを引用

Nagai, Y., Ando, T., Tanaka, T., & Kikuchi, Y. (2003). Recognition of tRNA bottom half by bacterial ribonuclease P.Nucleic acids research. Supplement (2001) (3 版, pp. 281-282)

Recognition of tRNA bottom half by bacterial ribonuclease P. / Nagai, Yasuhiro; Ando, Tomoaki; Tanaka, Terumichi; Kikuchi, Yo.

Nucleic acids research. Supplement (2001). 3. 編 2003. p. 281-282.

研究成果: Chapter

Nagai, Y, Ando, T, Tanaka, T & Kikuchi, Y 2003, Recognition of tRNA bottom half by bacterial ribonuclease P.Nucleic acids research. Supplement (2001). 3 Edn, pp. 281-282.
Nagai Y, Ando T, Tanaka T, Kikuchi Y. Recognition of tRNA bottom half by bacterial ribonuclease P. : Nucleic acids research. Supplement (2001). 3 版 2003. p. 281-282
Nagai, Yasuhiro ; Ando, Tomoaki ; Tanaka, Terumichi ; Kikuchi, Yo. / Recognition of tRNA bottom half by bacterial ribonuclease P. Nucleic acids research. Supplement (2001). 3. 版 2003. pp. 281-282
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