Recombinant human megakaryocyte growth and development factor (rhumgdf), a ligand for c‐mpl, produces functional human platelets in vitro

Esther S. Choi, Martha Hokom, Tim Bartley, Yue‐Sheng ‐S Li, Janet L. Nichol, Jim Skrine, Andrew Knudten, Janice Chen, Alex Hornkohl, Gustavo Grampp, Lisa Sleeman, Sean Cole, Geri Trail, Pamela Hunt*, Hideya Ohashi, Takashi Kato

*この研究の対応する著者

研究成果: Article査読

89 被引用数 (Scopus)

抄録

Platelet formation, occurring from bone marrow or lung megakaryocytes, has been difficult to study mechanistically. Recombinant human megakaryocyte growth and development factor (rHuMGDF), a recently described cytokine, has now been used to establish an in vitro system in which this important and little understood process occurs. CD34+ cells cultured with rHuMGDF develop into megakaryocytes which form long cytoplasmic extensions (proplatelets) that fragment into platelet‐sized particles (in vitro platelets). Morphologically, in vitro and human plasma‐derived platelets (control platelets) are virtually identical with respect to size, dense granule distribution and ultrastructural features. Functionally, in vitro and control platelets have similar aggregation and activation responses, and similarly incorporate mepacrine into dense granules. These findings suggest that rHuMGDF is sufficient to generate platelet‐synthesizing megakaryocytes from CD34+ cells and provide an experimental setting in which the study of human platelet formation can be adequately performed.

本文言語English
ページ(範囲)317-322
ページ数6
ジャーナルSTEM CELLS
13
3
DOI
出版ステータスPublished - 1995
外部発表はい

ASJC Scopus subject areas

  • 分子医療
  • 発生生物学
  • 細胞生物学

フィンガープリント

「Recombinant human megakaryocyte growth and development factor (rhumgdf), a ligand for c‐mpl, produces functional human platelets in vitro」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル