Recombinant human thrombopoietin (Mpl ligand) enhances proliferation of erythroid progenitors

Masao Kobayashi, Joseph H. Laver, Takashi Kato, Hiroshi Miyazaki, Makio Ogawa*

*この研究の対応する著者

研究成果: Article査読

153 被引用数 (Scopus)

抄録

We have studied the effects of recombinant human thrombopoietin (TPO, Mpl ligand) on human hematopoiesis in vitro. TPO alone did not support erythroid burst formation but, in the presence of erythropoietin, it enhanced erythroid burst formation from CD34+ bone marrow and cord blood cells. The burst- promoting activity (BPA) was stronger under 5% serum than 30% serum conditions. The direct nature of BPA effects was documented by replating studies of early erythroid bursts. The BPA of TPO was less than that of interleukin-3 but was comparable with that of granulocyte/macrophage colony- stimulating factor and steel factor. The soluble form of Mpl receptor inhibited burst enhancing effects of TPO, suggesting that the BPA effects of TPO are mediated through the Mpl receptor. These results further delineate the physiologic roles of TPO and may aid in the determination of the clinical usages of TPO.

本文言語English
ページ(範囲)2494-2499
ページ数6
ジャーナルBlood
86
7
DOI
出版ステータスPublished - 1995 10 1
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 免疫学
  • 血液学
  • 細胞生物学

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