Reconstitution of 30S ribosomal subunits in vitro using ribosome biogenesis factors

Daichi Tamaru, Kazuaki Amikura*, Yoshihiro Shimizu, Knud H. Nierhaus, Takuya Ueda

*この研究の対応する著者

研究成果: Article査読

14 被引用数 (Scopus)

抄録

Reconstitution of ribosomes in vitro from individual ribosomal proteins provides a powerful tool for understanding the ribosome assembly process including the sequential incorporation of ribosomal proteins. However, conventional assembly methods require high-salt conditions for efficient ribosome assembly. In this study, we reconstituted 30S ribosomal subunits from individually purified ribosomal proteins in the presence of ribosome biogenesis factors. In this system, two GTPases (Era and YjeQ) facilitated assembly of a 30S subunit exhibiting poly(U)-directed polyphenylalanine synthesis and native protein synthesis under physiological conditions. This in vitro system permits a study of the assembly process and function of ribosome biogenesis factors, and it will facilitate the generation of ribosomes from DNA without using cells.

本文言語English
ページ(範囲)1512-1519
ページ数8
ジャーナルRNA
24
11
DOI
出版ステータスPublished - 2018 11月
外部発表はい

ASJC Scopus subject areas

  • 分子生物学

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