Reconstitution of circadian oscillation of cyanobacterial KaiC phosphorylation in vitro

Masato Nakajima, Keiko Imai, Hiroshi Ito, Taeko Nishiwaki, Yoriko Murayama, Hideo Iwasaki, Tokitaka Oyama, Takao Kondo*

*この研究の対応する著者

研究成果: Article査読

759 被引用数 (Scopus)

抄録

Kai proteins globally regulate circadian gene expression of cyanobacteria. The KaiC phosphorylation cycle, which persists even without transcription or translation, is assumed to be a basic timing process of the circadian clock. We have reconstituted the self-sustainable oscillation of KaiC phosphorylation in vitro by incubating KaiC with KaiA, KaiB, and adenosine triphosphate. The period of the in vitro oscillation was stable despite temperature change (temperature compensation), and the circadian periods observed in vivo in KaiC mutant strains were consistent with those measured in vitro. The enigma of the circadian clock can now be studied in vitro by examining the interactions between three Kai proteins.

本文言語English
ページ(範囲)414-415
ページ数2
ジャーナルScience
308
5720
DOI
出版ステータスPublished - 2005 4月 15
外部発表はい

ASJC Scopus subject areas

  • 一般

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