抜粋
It was recently demonstrated that peptide bond formation can occur using an Escherichia coli naked 23S ribosomal RNA without any of the ribosomal proteins. Here, the six domains of the 23S ribosomal RNA were individually synthesized and shown to be capable, when complexed together, of stimulating the reaction. Omission and addition experiments indicated that the activity could be reconstituted solely by domain V at a concentration 10 times higher than that of the intact 23S ribosomal RNA, whereas domain VI could enhance the activity in trans. These findings suggest that fragments of an RNA molecule have the ability to associate into a functional whole.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 666-669 |
| ページ数 | 4 |
| ジャーナル | Science |
| 巻 | 281 |
| 発行部数 | 5377 |
| DOI | |
| 出版物ステータス | Published - 1998 7 31 |
| 外部発表 | Yes |
ASJC Scopus subject areas
- General
フィンガープリント Reconstitution of peptide bond formation with Escherichia coli 23S ribosomal RNA domains' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。
これを引用
Nitta, I., Kamada, Y., Noda, H., Ueda, T., & Watanabe, K. (1998). Reconstitution of peptide bond formation with Escherichia coli 23S ribosomal RNA domains. Science, 281(5377), 666-669. https://doi.org/10.1126/science.281.5377.666