Reconstitution of peptide bond formation with Escherichia coli 23S ribosomal RNA domains

Itaru Nitta, Yoshie Kamada, Hiroe Noda, Takuya Ueda, Kimitsuna Watanabe*

*この研究の対応する著者

研究成果: Article査読

61 被引用数 (Scopus)

抄録

It was recently demonstrated that peptide bond formation can occur using an Escherichia coli naked 23S ribosomal RNA without any of the ribosomal proteins. Here, the six domains of the 23S ribosomal RNA were individually synthesized and shown to be capable, when complexed together, of stimulating the reaction. Omission and addition experiments indicated that the activity could be reconstituted solely by domain V at a concentration 10 times higher than that of the intact 23S ribosomal RNA, whereas domain VI could enhance the activity in trans. These findings suggest that fragments of an RNA molecule have the ability to associate into a functional whole.

本文言語English
ページ(範囲)666-669
ページ数4
ジャーナルScience
281
5377
DOI
出版ステータスPublished - 1998 7月 31
外部発表はい

ASJC Scopus subject areas

  • 一般

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