Reconstruction of a Vascular Bed with Perfusable Blood Vessels Using a Decellularized Porcine Small Intestine for Clinical Application

Yusuke Tobe, Katsuhisa Sakaguchi, Jun Homma, Kazunori Sano, Eiji Kobayashi, Hidekazu Sekine, Kiyotaka Iwasaki, Tatsuya Shimizu, Mitsuo Umezu

研究成果: Conference contribution

抄録

Engineering three-dimensional tissues in vitro for regenerative therapy is highly desired. Vascular beds with connectable arteries and veins play crucial roles in fabricating three-dimensional tissues in vitro and improving the survival rate of transplanted three-dimensional tissues in vivo. Here, we developed a method to reconstruct a new vascular bed that could be implanted into humans for clinical applications. Porcine small intestine with an arteriovenous loop was selected as a vascular bed skeleton and decellularized using transvascular perfusion with both detergent and enzyme. Subsequently, the decellularized intestinal graft was re-endothelialized with human cells to allow blood perfusion into the vascular bed. The small intestinal graft was successfully decellularized without severe damage to the burst pressure of the graft, and the decellularized intestinal graft could be transplanted without a severe inflammatory response in rats for two weeks. In addition, the decellularized intestinal graft was partially vascularized with human endothelial cells in seven days. Therefore, the reconstructed intestinal vascular bed may serve as a human transplantable vascular bed with potential in clinical investigations.

本文言語English
ホスト出版物のタイトル11th Asian-Pacific Conference on Medical and Biological Engineering - Proceedings of the Online Conference, APCMBE 2020
編集者Yasuyuki Shiraishi, Ichiro Sakuma, Keiji Naruse, Akinori Ueno
出版社Springer Science and Business Media Deutschland GmbH
ページ284-292
ページ数9
ISBN(印刷版)9783030661687
DOI
出版ステータスPublished - 2021
イベント11th Asian-Pacific Conference on Medical and Biological Engineering, APCMBE 2020 - Okayama, Japan
継続期間: 2020 5 252020 5 27

出版物シリーズ

名前IFMBE Proceedings
82
ISSN(印刷版)1680-0737
ISSN(電子版)1433-9277

Conference

Conference11th Asian-Pacific Conference on Medical and Biological Engineering, APCMBE 2020
国/地域Japan
CityOkayama
Period20/5/2520/5/27

ASJC Scopus subject areas

  • バイオエンジニアリング
  • 生体医工学

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