Cell-cell communication between multiple cell populations is believed to be important for the activation of many cellular functions. It has previously been reported that rat hepatocytes in a double layer co-culture system with human endothelial cells were able to maintain albumin expression for a prolonged period of time. This result suggests that essential functions of hepatocytes were maintained via communication with endothelial cells in this co-culture system. In order to analyze the hepatocyte gene expression profile of double-layered co-culture, we try to isolate cDNAs, which increase in this co-culture system, from a subtractive library constructed by suppression subtractive hybridization (SSH). The results indicate that the many hepatocyte functions are increased by layered with HUVEC.
|出版ステータス||Published - 2006 12 1|
|イベント||55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan|
継続期間: 2006 9 20 → 2006 9 22
|Other||55th Society of Polymer Science Japan Symposium on Macromolecules|
|Period||06/9/20 → 06/9/22|
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