Reduction-triggered fluorescence probe for peptide-templated reactions

Aya Shibata, Hiroshi Abe, Kazuhiro Furukawa, Satoshi Tsuneda, Yoshihiro Ito

研究成果: Article

5 引用 (Scopus)

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We developed a new nucleic acid-based fluorescence probe for protein detection. The method is based on the scission of an aptamer into two probes, which are then attached with a chemically reactive fluorogenic compound. The protein-dependent association of the two probes accelerates a reduction-triggered fluorogenic reaction and indicates the presence of the target protein, which is detected using a fluorescence readout. The fluorescence signal is generated via the deprotection of the azidomethyl group of fluorescein. The arginine-rich motif peptide of the human immunodeficiency virus-1 Rev protein was targeted by this type of probe. Emission was detected at 522 nm and was enhanced by about 19.4-fold in the presence of the target peptide. An oligonucleotide-based reduction-triggered fluorescence probe was successfully applied to the detection of the Rev peptide in solution.

元の言語English
ページ(範囲)1223-1226
ページ数4
ジャーナルChemical and Pharmaceutical Bulletin
57
発行部数11
DOI
出版物ステータスPublished - 2009 11 1

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ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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