Reduction-triggered fluorescence probe for peptide-templated reactions

Aya Shibata, Hiroshi Abe*, Kazuhiro Furukawa, Satoshi Tsuneda, Yoshihiro Ito

*この研究の対応する著者

研究成果査読

5 被引用数 (Scopus)

抄録

We developed a new nucleic acid-based fluorescence probe for protein detection. The method is based on the scission of an aptamer into two probes, which are then attached with a chemically reactive fluorogenic compound. The protein-dependent association of the two probes accelerates a reduction-triggered fluorogenic reaction and indicates the presence of the target protein, which is detected using a fluorescence readout. The fluorescence signal is generated via the deprotection of the azidomethyl group of fluorescein. The arginine-rich motif peptide of the human immunodeficiency virus-1 Rev protein was targeted by this type of probe. Emission was detected at 522 nm and was enhanced by about 19.4-fold in the presence of the target peptide. An oligonucleotide-based reduction-triggered fluorescence probe was successfully applied to the detection of the Rev peptide in solution.

本文言語English
ページ(範囲)1223-1226
ページ数4
ジャーナルChemical and Pharmaceutical Bulletin
57
11
DOI
出版ステータスPublished - 2009 11

ASJC Scopus subject areas

  • 化学 (全般)
  • 創薬

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