Reduction-triggered fluorescence probe for peptide-templated reactions

Aya Shibata, Hiroshi Abe, Kazuhiro Furukawa, Satoshi Tsuneda, Yoshihiro Ito

    研究成果: Article

    5 引用 (Scopus)

    抄録

    We developed a new nucleic acid-based fluorescence probe for protein detection. The method is based on the scission of an aptamer into two probes, which are then attached with a chemically reactive fluorogenic compound. The protein-dependent association of the two probes accelerates a reduction-triggered fluorogenic reaction and indicates the presence of the target protein, which is detected using a fluorescence readout. The fluorescence signal is generated via the deprotection of the azidomethyl group of fluorescein. The arginine-rich motif peptide of the human immunodeficiency virus-1 Rev protein was targeted by this type of probe. Emission was detected at 522 nm and was enhanced by about 19.4-fold in the presence of the target peptide. An oligonucleotide-based reduction-triggered fluorescence probe was successfully applied to the detection of the Rev peptide in solution.

    元の言語English
    ページ(範囲)1223-1226
    ページ数4
    ジャーナルChemical and Pharmaceutical Bulletin
    57
    発行部数11
    DOI
    出版物ステータスPublished - 2009 11

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    Fluorescence
    Peptides
    Proteins
    Fluorescein
    Oligonucleotides
    Nucleic Acids
    Arginine
    Association reactions

    ASJC Scopus subject areas

    • Drug Discovery
    • Chemistry(all)

    これを引用

    Reduction-triggered fluorescence probe for peptide-templated reactions. / Shibata, Aya; Abe, Hiroshi; Furukawa, Kazuhiro; Tsuneda, Satoshi; Ito, Yoshihiro.

    :: Chemical and Pharmaceutical Bulletin, 巻 57, 番号 11, 11.2009, p. 1223-1226.

    研究成果: Article

    Shibata, Aya ; Abe, Hiroshi ; Furukawa, Kazuhiro ; Tsuneda, Satoshi ; Ito, Yoshihiro. / Reduction-triggered fluorescence probe for peptide-templated reactions. :: Chemical and Pharmaceutical Bulletin. 2009 ; 巻 57, 番号 11. pp. 1223-1226.
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    AU - Ito, Yoshihiro

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