Reentrant conformational transitions in DNA molecules complexed with cationic surfactant

S. V. Mikhailenko; V. G. Sergeyev; K. Yoshikawa

Reentrant conformational transitions in DNA molecules complexed with cationic surfactant

S. V. Mikhailenko, V. G. Sergeyev, K. Yoshikawa

研究成果: Paper › 査読

抄録

We have established the conditions for the dissociation-formation of DNA-CTAB complexes in organic solvents of different polarity. Fluorescence microscopy and CD spectroscopy indicate that DNA-CTAB complexes dissociate into their initial components at intermediate concentrations of alcohol, while conserving the double-stranded structure of native DNA. A further increase in the alcohol concentration leads to restoration of the complex, which is soluble under these conditions, i.e. in low-polarity organic media. As a novel phenomenon on DNA-CTAB interacting system, we have found the alcohol-induced reentrant globule-coil-globule transition in T4DNA-CTAB complexes.

本文言語	English
ページ	213-215
ページ数	3
出版ステータス	Published - 1998 12 1
外部発表	はい
イベント	Proceedings of the 1998 9th International Symposium on Micromechatronics and Human Science, MHS - Nagoya, Jpn 継続期間: 1998 11 25 → 1998 11 28

Other

Other	Proceedings of the 1998 9th International Symposium on Micromechatronics and Human Science, MHS
City	Nagoya, Jpn
Period	98/11/25 → 98/11/28

ASJC Scopus subject areas

Engineering(all)

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引用スタイル

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AU - Mikhailenko, S. V.

AU - Sergeyev, V. G.

AU - Yoshikawa, K.

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N2 - We have established the conditions for the dissociation-formation of DNA-CTAB complexes in organic solvents of different polarity. Fluorescence microscopy and CD spectroscopy indicate that DNA-CTAB complexes dissociate into their initial components at intermediate concentrations of alcohol, while conserving the double-stranded structure of native DNA. A further increase in the alcohol concentration leads to restoration of the complex, which is soluble under these conditions, i.e. in low-polarity organic media. As a novel phenomenon on DNA-CTAB interacting system, we have found the alcohol-induced reentrant globule-coil-globule transition in T4DNA-CTAB complexes.

AB - We have established the conditions for the dissociation-formation of DNA-CTAB complexes in organic solvents of different polarity. Fluorescence microscopy and CD spectroscopy indicate that DNA-CTAB complexes dissociate into their initial components at intermediate concentrations of alcohol, while conserving the double-stranded structure of native DNA. A further increase in the alcohol concentration leads to restoration of the complex, which is soluble under these conditions, i.e. in low-polarity organic media. As a novel phenomenon on DNA-CTAB interacting system, we have found the alcohol-induced reentrant globule-coil-globule transition in T4DNA-CTAB complexes.

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