Region and amino acid residues required for Rad51C binding in the human Xrcc3 protein

Hitoshi Kurumizaka, Rima Enomoto, Maki Nakada, Keiko Eda, Shigeyuki Yokoyama, Takehiko Shibata*

*この研究の対応する著者

    研究成果査読

    16 被引用数 (Scopus)

    抄録

    The Xrcc3 protein, which is required for the homologous recombinational repair of damaged DNA, forms a complex with the Rad51C protein in human cells. Mutations in either the Xrcc3 or Rad51C gene cause extreme sensitivity to DNA-damaging agents and generate the genomic instability frequently found in tumors. In the present study, we found that the Xrcc3 segment containing amino acid residues 63-346, Xrcc363-346, is the Rad51C-binding region. Biochemical analyses revealed that Xrcc363-346 forms a complex with Rad51C, and the Xrcc363-346-Rad51C complex possesses ssDNA and dsDNA binding abilities comparable to those of the full-length Xrcc3-Rad51C complex. Based on the structure of RecA, which is thought to be the ancestor of Xrcc3, six Xrcc3 point mutants were designed. Two-hybrid and biochemical analyses of the Xrcc3 point mutants revealed that Tyr139 and Phe249 are essential amino acid residues for Rad51C binding. Superposition of the Xrcc3 Tyr139 and Phe249 residues on the RecA structure suggested that Tyr139 may function to ensure proper folding and Phe249 may be important to constitute the Rad51C-binding interface in Xrcc3.

    本文言語English
    ページ(範囲)4041-4050
    ページ数10
    ジャーナルNucleic Acids Research
    31
    14
    DOI
    出版ステータスPublished - 2003 7 15

    ASJC Scopus subject areas

    • 遺伝学

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