Regulation of ribozyme activity by engineered protein switch.

Terumichi Tanaka, Naomi Kanda, Yo Kikuchi

研究成果: Article査読

抄録

Previously, we showed that the P3 domain of the Escherichia coli ribonuclease P ribozyme can be truncated and replaced in vitro. In this study, we prepared a P3-replaced variant of the E. coli ribozyme that has HIV TAR sequence as the engineered P3 domain. The mutant ribozyme demonstrated the ribonuclease P activity and was inhibited in the presence of the HIV tat protein fragment. Our results showed that the P3 domain of this enzyme can be engineered, and addition of some heterologous protein subunits can also be done to this domain.

本文言語English
ページ(範囲)343-344
ページ数2
ジャーナルNucleic acids symposium series (2004)
49
出版ステータスPublished - 2005
外部発表はい

ASJC Scopus subject areas

  • 医学(全般)

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