Regulatory roles of MgADP and calcium in tension development of skinned cardiac muscle

Norio Fukuda, Hideaki Fujita, Takashi Fujita, Shin'ichi Ishiwata*

*この研究の対応する著者

    研究成果: Article査読

    41 被引用数 (Scopus)

    抄録

    We investigated the regulatory roles of MgADP and free Ca2+ in isometric tension development in skinned bovine cardiac muscle. We found that, in the relaxed state without free Ca2+, MgADP elicited a sigmoidal increase in active tension, as is the case in skeletal muscle (ADP-contraction). The critical MgADP concentration, at which the tension increment became half-maximal, increased in proportion to MgATP concentration, with a slope of approximately 1 for cardiac and 4 for skeletal muscle. Raising the free Ca2+ concentration decreased the critical MgADP concentration in proportion to the free Ca2+ concentration. In addition, the apparent Ca2+ sensitivity of tension development increased with MgADP, while decreasing with inorganic phosphate (Pi); MgADP suppressed the Ca2+-desensitizing effect of Pi in a concentration-dependent manner. These activating effects of MgADP were quantitatively assessed by means of a model based upon the kinetic scheme of actomyosin ATPase. These experimental results and model simulation suggest that the state of thin filaments is synergistically regulated by both the binding of Ca2+ to troponin and the formation of the actomyosin-ADP complex.

    本文言語English
    ページ(範囲)909-921
    ページ数13
    ジャーナルJournal of Muscle Research and Cell Motility
    19
    8
    DOI
    出版ステータスPublished - 1998

    ASJC Scopus subject areas

    • 生理学
    • 臨床生化学
    • 内分泌学
    • 細胞生物学

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