Relation between effective charge numbers and signals caused by protein adsorption on field effect transistor detection

S. Hideshima*, T. Nakamura, S. Kuroiwa, T. Osaka

*この研究の対応する著者

研究成果: Conference contribution

3 被引用数 (Scopus)

抄録

This paper describes a practical method for predicting signals of an adsorbed protein detected by field effect transistors (FETs). The FETs detect the change of the surface charge caused by protein adsorption. The sensing signals strongly depend on an ionic strength of the buffer solution. The prediction of signals is useful for optimizing the strength. A calculation method using three-dimensional conformation structure of proteins was proposed for the prediction tool in the latest paper citing biotin-avidin interaction as an example. To verify availability of the method for alternative adsorbed protein, an adsorption model of streptavidin, which also has a binding ability to biotin, was investigated. Streptavidin has little charge in a buffer solution at pH 7.4 calculated from the method. The results suggest that the signals caused by adsorption of streptavidin were barely detectable using a biotinylated FET.

本文言語English
ホスト出版物のタイトルBioelectronics, Biointerfaces, and Biomedical Applications 4
出版社Electrochemical Society Inc.
ページ121-124
ページ数4
7
ISBN(電子版)9781566778688
ISBN(印刷版)9781607682189
DOI
出版ステータスPublished - 2011

出版物シリーズ

名前ECS Transactions
番号7
35
ISSN(印刷版)1938-5862
ISSN(電子版)1938-6737

ASJC Scopus subject areas

  • 工学(全般)

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