Repeated batch production of L‐phenylalanine from phenylpyruvate and NH4Cl by immobilized cells of Nocardia opaca under hydrogen high pressure

Tadashi Matsunaga, Michio Higashijima, Anny Sulaswatty, Shigeo Nishimura, Takanori Kitamura, Masao Tsuji, Toshio Kawaguchi

研究成果: Article

9 引用 (Scopus)

抄録

Among various microbial cells examined under screening conditions, Nocardia opaca showed the highest activity for production of phenylalanine from phenylpyruvate. Here NH4Cl as well as amino acids were used as an amino donor for phenylalanine production. The phenylalanine production rate increased with increasing hydrogen pressure. The specific activity of phenylalanine dehydrogenase was increased by culturing N. opaca cells in nutrient broth containing 0.3% phenylalanine. As a result, the phenylalanine production rate increased from 0.69 to 4.4 μmol/min g dry cells. Immobilized cells were activated in nutrient broth containing ZnCl2 before phenylalanine production. Phenylalanine dehydrogenase activity and cell number in the gel increased with increasing incubation time, and the maximum phenylalanine dehydrogenase activity was obtained at 36 h incubation. Then, phenylalanine was produced from phenylpyruvate, NH4Cl, and 100 atm H2 with the activated immobilized cells. The rate of phenylalanine production was 0.24 μmol/min cm3 gel. The conversion of phenylpyruvate to phenylalanine was 82%. Immobilized cells retained 76% of the initial phenylalanine production rate after 10 h reactions were repeated 11 times with two intervening reactivations.

元の言語English
ページ(範囲)834-840
ページ数7
ジャーナルBiotechnology and Bioengineering
31
発行部数8
DOI
出版物ステータスPublished - 1988
外部発表Yes

Fingerprint

Nocardia
Immobilized Cells
Phenylalanine
Hydrogen
Cells
Pressure
Nutrients
Gels
phenylpyruvic acid
Food
Amino acids
Screening
Cell Count
Amino Acids

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Repeated batch production of L‐phenylalanine from phenylpyruvate and NH4Cl by immobilized cells of Nocardia opaca under hydrogen high pressure. / Matsunaga, Tadashi; Higashijima, Michio; Sulaswatty, Anny; Nishimura, Shigeo; Kitamura, Takanori; Tsuji, Masao; Kawaguchi, Toshio.

:: Biotechnology and Bioengineering, 巻 31, 番号 8, 1988, p. 834-840.

研究成果: Article

Matsunaga, Tadashi ; Higashijima, Michio ; Sulaswatty, Anny ; Nishimura, Shigeo ; Kitamura, Takanori ; Tsuji, Masao ; Kawaguchi, Toshio. / Repeated batch production of L‐phenylalanine from phenylpyruvate and NH4Cl by immobilized cells of Nocardia opaca under hydrogen high pressure. :: Biotechnology and Bioengineering. 1988 ; 巻 31, 番号 8. pp. 834-840.
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abstract = "Among various microbial cells examined under screening conditions, Nocardia opaca showed the highest activity for production of phenylalanine from phenylpyruvate. Here NH4Cl as well as amino acids were used as an amino donor for phenylalanine production. The phenylalanine production rate increased with increasing hydrogen pressure. The specific activity of phenylalanine dehydrogenase was increased by culturing N. opaca cells in nutrient broth containing 0.3{\%} phenylalanine. As a result, the phenylalanine production rate increased from 0.69 to 4.4 μmol/min g dry cells. Immobilized cells were activated in nutrient broth containing ZnCl2 before phenylalanine production. Phenylalanine dehydrogenase activity and cell number in the gel increased with increasing incubation time, and the maximum phenylalanine dehydrogenase activity was obtained at 36 h incubation. Then, phenylalanine was produced from phenylpyruvate, NH4Cl, and 100 atm H2 with the activated immobilized cells. The rate of phenylalanine production was 0.24 μmol/min cm3 gel. The conversion of phenylpyruvate to phenylalanine was 82{\%}. Immobilized cells retained 76{\%} of the initial phenylalanine production rate after 10 h reactions were repeated 11 times with two intervening reactivations.",
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AU - Matsunaga, Tadashi

AU - Higashijima, Michio

AU - Sulaswatty, Anny

AU - Nishimura, Shigeo

AU - Kitamura, Takanori

AU - Tsuji, Masao

AU - Kawaguchi, Toshio

PY - 1988

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N2 - Among various microbial cells examined under screening conditions, Nocardia opaca showed the highest activity for production of phenylalanine from phenylpyruvate. Here NH4Cl as well as amino acids were used as an amino donor for phenylalanine production. The phenylalanine production rate increased with increasing hydrogen pressure. The specific activity of phenylalanine dehydrogenase was increased by culturing N. opaca cells in nutrient broth containing 0.3% phenylalanine. As a result, the phenylalanine production rate increased from 0.69 to 4.4 μmol/min g dry cells. Immobilized cells were activated in nutrient broth containing ZnCl2 before phenylalanine production. Phenylalanine dehydrogenase activity and cell number in the gel increased with increasing incubation time, and the maximum phenylalanine dehydrogenase activity was obtained at 36 h incubation. Then, phenylalanine was produced from phenylpyruvate, NH4Cl, and 100 atm H2 with the activated immobilized cells. The rate of phenylalanine production was 0.24 μmol/min cm3 gel. The conversion of phenylpyruvate to phenylalanine was 82%. Immobilized cells retained 76% of the initial phenylalanine production rate after 10 h reactions were repeated 11 times with two intervening reactivations.

AB - Among various microbial cells examined under screening conditions, Nocardia opaca showed the highest activity for production of phenylalanine from phenylpyruvate. Here NH4Cl as well as amino acids were used as an amino donor for phenylalanine production. The phenylalanine production rate increased with increasing hydrogen pressure. The specific activity of phenylalanine dehydrogenase was increased by culturing N. opaca cells in nutrient broth containing 0.3% phenylalanine. As a result, the phenylalanine production rate increased from 0.69 to 4.4 μmol/min g dry cells. Immobilized cells were activated in nutrient broth containing ZnCl2 before phenylalanine production. Phenylalanine dehydrogenase activity and cell number in the gel increased with increasing incubation time, and the maximum phenylalanine dehydrogenase activity was obtained at 36 h incubation. Then, phenylalanine was produced from phenylpyruvate, NH4Cl, and 100 atm H2 with the activated immobilized cells. The rate of phenylalanine production was 0.24 μmol/min cm3 gel. The conversion of phenylpyruvate to phenylalanine was 82%. Immobilized cells retained 76% of the initial phenylalanine production rate after 10 h reactions were repeated 11 times with two intervening reactivations.

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