Repression of the activities of two extracytoplasmic function σ factors, σM and σV, of Bacillus subtilis by glucolipids in Escherichia coli cells

Takahiro Seki, Ryota Mineshima, Michihiro Hashimoto, Kouji Matsumoto, Hiroshi Hara*, Satoshi Matsuoka

*この研究の対応する著者

研究成果: Article査読

6 被引用数 (Scopus)

抄録

Extracytoplasmic function (ECF) σ factors respond to environmental stresses and regulate numerous genes required for adaptation. Under normal growth conditions, the ECF σ factors are sequestered by transmembrane anti-σ factor proteins, from which they are released under stress conditions. In Bacillus subtilis ugtP null mutant cells, which lack glucolipids, three of the seven ECF σ factors, σM, σV and σX, are activated. The Escherichia coli cell membrane does not contain glucolipids. When the genes for these three ECF σ and anti-σ factors were introduced into E. coli cells, expression of lacZ fused to the ECF σ factor-regulated promoters indicated ECF σ factor activity. Additional expression of the ugtP gene in these E. coli cells led to the synthesis of small amounts of glucolipids, and the activities of σM and σV were repressed, but the activity of σX was unaffected. It is likely that glucolipids directly influence anti-σM and anti-σV factors by stabilizing conformations that sequester the respective ECF σ factors.

本文言語English
ページ(範囲)109-114
ページ数6
ジャーナルGenes and Genetic Systems
90
2
DOI
出版ステータスPublished - 2015 9月 18
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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