TY - JOUR
T1 - Reproducibility of fluorescent expression from engineered biological constructs in E. coli
AU - iGEM Interlab Study Contributors
AU - Beal, Jacob
AU - Haddock-Angelli, Traci
AU - Gershater, Markus
AU - De Mora, Kim
AU - Lizarazo, Meagan
AU - Hollenhorst, Jim
AU - Rettberg, Randy
AU - Demling, Philipp
AU - Hanke, Rene
AU - Osthege, Michael
AU - Schechtel, Anna
AU - Sudarsan, Suresh
AU - Zimmermann, Arne
AU - Gabryelczyk, Bartosz
AU - Ikonen, Martina
AU - Salmela, Minnamari
AU - Acar, Muradýye
AU - Aktas, Muhammed Fatih
AU - Bestepe, Furkan
AU - Ceylan, Furkan Sacit
AU - Cigdem, Sadýk
AU - Dohan, Mikail
AU - Elitok, Mustafa
AU - Gunduz, Mehmet
AU - Gunduz, Esra
AU - Hatipoglu, Omer Faruk
AU - Kaya, Turan
AU - Sayin, Orhan
AU - Tapan, Safa
AU - Tereci, Osman Faruk
AU - Uçar, Abdullah
AU - Yilmaz, Mustafa
AU - Barrick, Jeffrey
AU - Gutierrez, Alex
AU - Mishler, Dennis
AU - Monk, Jordan
AU - Mortensen, Kate
AU - Shin, Nathan
AU - Watkins, Ella
AU - Chen, Yintong
AU - Jin, Yuji
AU - Shi, Yuanjie
AU - Zhang, Haoqian Myelin
AU - Ono, Bruno
AU - Ribovski, Lais
AU - Silva, Ivan
AU - Zampronio, Danilo Keiji
AU - Birkholz, Nils
AU - Busche, Rudiger Frederik
AU - Kiga, Daisuke
PY - 2016/3
Y1 - 2016/3
N2 - We present results of the first large-scale interlaboratory study carried out in synthetic biology, as part of the 2014 and 2015 International Genetically Engineered Machine (iGEM) competitions. Participants at 88 institutions around the world measured fluorescence from three engineered constitutive constructs in E. coli. Few participants were able to measure absolute fluorescence, so data was analyzed in terms of ratios. Precision was strongly related to fluorescent strength, ranging from 1.54-fold standard deviation for the ratio between strong promoters to 5.75-fold for the ratio between the strongest and weakest promoter, and while host strain did not affect expression ratios, choice of instrument did. This result shows that high quantitative precision and reproducibility of results is possible, while at the same time indicating areas needing improved laboratory practices.
AB - We present results of the first large-scale interlaboratory study carried out in synthetic biology, as part of the 2014 and 2015 International Genetically Engineered Machine (iGEM) competitions. Participants at 88 institutions around the world measured fluorescence from three engineered constitutive constructs in E. coli. Few participants were able to measure absolute fluorescence, so data was analyzed in terms of ratios. Precision was strongly related to fluorescent strength, ranging from 1.54-fold standard deviation for the ratio between strong promoters to 5.75-fold for the ratio between the strongest and weakest promoter, and while host strain did not affect expression ratios, choice of instrument did. This result shows that high quantitative precision and reproducibility of results is possible, while at the same time indicating areas needing improved laboratory practices.
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U2 - 10.1371/journal.pone.0150182
DO - 10.1371/journal.pone.0150182
M3 - Article
C2 - 26937966
AN - SCOPUS:84961783220
VL - 11
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 3
M1 - e0150182
ER -