Resolving stepping rotation in Thermus thermophilus H+-ATPase/ synthase with an essentially drag-free probe

Shou Furuike, Masahiro Nakano, Kengo Adachi, Hiroyuki Noji, Kazuhiko Kinosita*, Ken Yokoyama

*この研究の対応する著者

    研究成果査読

    37 被引用数 (Scopus)

    抄録

    Vacuole-type ATPases (VoV1) and FoF 1 ATP synthases couple ATP hydrolysis/synthesis in the soluble V 1 or F1 portion with proton (or Na+) flow in the membrane-embedded Vo or Fo portion through rotation of one common shaft. Here we show at submillisecond resolutions the ATP-driven rotation of isolated V1 and the whole VoV1 from Thermus thermophilus, by attaching a 40-nm gold bead for which viscous drag is almost negligible. V1 made 120 ° steps, commensurate with the presence of three catalytic sites. Dwells between the steps involved at least two events other than ATP binding, one likely to be ATP hydrolysis. V oV1 exhibited 12 dwell positions per revolution, consistent with the 12-fold symmetry of the Vo rotor in T. thermophilus. Unlike F 1 that undergoes 80 °-40 ° substepping, chemo-mechanical checkpoints in isolated V1 are all at the ATP-waiting position, and Vo adds further bumps through stator-rotor interactions outside and remote from V1.

    本文言語English
    論文番号233
    ジャーナルNature Communications
    2
    1
    DOI
    出版ステータスPublished - 2011

    ASJC Scopus subject areas

    • 生化学、遺伝学、分子生物学(全般)
    • 化学 (全般)
    • 物理学および天文学(全般)

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