Response of Saccharomyces cerevisiae to a monoterpene: Evaluation of antifungal potential by DNA microarray analysis

Meher Parveen, Md Kamrul Hasan, Junko Takahashi, Yoshinori Murata, Emiko Kitagawa, Osamu Kodama, Hitoshi Iwahashi*

*この研究の対応する著者

研究成果: Article査読

83 被引用数 (Scopus)

抄録

Plant-derived essential oils with monoterpenoids have been used as antifungal drugs since ancient times, but the mode of action of these natural hydrocarbons at the molecular level is not understood. In order to understand the mechanisms of toxicity of α-terpinene (a cyclic monoterpene), a culture of Saccharomyces cerevisiae was exposed to 0.02% α-terpinene for 2 h and transcript profiles were obtained using yeast DNA arrays. These profiles, when compared with transcript profiles of untreated cultures, revealed that the expression of 793 genes was affected. For 435 genes, mRNA levels in treated cells compared with control cells differed by more than two-fold, whereas for 358 genes, it was <0.5-fold. Northern blots were performed for selected genes to verify the microarray results. Functional analysis of the up-regulated genes indicates that, similar to commonly used antifungal drugs, α -terpinene exposure affected genes involved in ergosterol biosynthesis and sterol uptake. In addition, transcriptional induction of genes related to lipid metabolism, cell wall structure and function, detoxification and cellular transport was observed in response to terpinene toxicity. Notably, thefunctions of 192 up-regulated genes arestill unknown, but their characterization will probably shed light on the mechanisms of drug resistance and sensitivity. Taken together, this study showed that α-terpinene has strong antifungal activities and its modes of action resemble those of presently used antifungal drugs.

本文言語English
ページ(範囲)46-55
ページ数10
ジャーナルJournal of Antimicrobial Chemotherapy
54
1
DOI
出版ステータスPublished - 2004 7
外部発表はい

ASJC Scopus subject areas

  • 薬理学
  • 微生物学(医療)
  • 感染症
  • 薬理学(医学)

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