Reversible and irreversible modification of erythrocyte membrane permeability by electric field

Engin H. Serpersu, Kazuhiko Kinosita, Yow Tsong Tian Yow Tsong*

*この研究の対応する著者

研究成果: Article査読

112 被引用数 (Scopus)

抄録

Electric fields of a few kV/cm and of duration in microseconds are known to implant pores of limited size in cell membranes. We report here a study of kinetics of pore formation and reversibility of pores. Loading of biologically active molecules was also attempted. For human erythrocytes in an isotonic saline, pores allowed passive Rb+ entry formed within 0.5 μs when a 4 kV/cm electric pulse was used. Pores that admitted oligosaccharides were introduced with an electric pulse of a longer duration in an isosmotic mixture of NaCl and sucrose. These pores were irreversible under most circumstances, but they could be resealed in an osmotically balanced medium. A complete resealing of pores that admitted Rb+ took approximately 40 min at 37°C. Resealing of pores that admitted sucrose took much longer, 20 h, under similar conditions. In other cell types, resealing step may be omitted due to stronger membrane structures. Experimental protocols for loading small molecules into cells without losing cytoplasmic macromolecules are discussed.

本文言語English
ページ(範囲)779-785
ページ数7
ジャーナルBBA - Biomembranes
812
3
DOI
出版ステータスPublished - 1985 2 14
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 生物理学
  • 細胞生物学

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