RGB-Color Intensiometric Indicators to Visualize Spatiotemporal Dynamics of ATP in Single Cells

Satoshi Arai, Rókus Kriszt, Kazuki Harada, Liang Sheng Looi, Shogo Matsuda, Devina Wongso, Satoshi Suo, Shoichi Ishiura, Yu Hua Tseng, Michael Raghunath, Toshiro Ito, Takashi Tsuboi, Tetsuya Kitaguchi

研究成果: Article査読

25 被引用数 (Scopus)

抄録

Adenosine triphosphate (ATP) provides energy for the regulation of multiple cellular processes in living organisms. Capturing the spatiotemporal dynamics of ATP in single cells is fundamental to our understanding of the mechanisms underlying cellular energy metabolism. However, it has remained challenging to visualize the dynamics of ATP in and between distinct intracellular organelles and its interplay with other signaling molecules. Using single fluorescent proteins, multicolor ATP indicators were developed, enabling the simultaneous visualization of subcellular ATP dynamics in the cytoplasm and mitochondria of cells derived from mammals, plants, and worms. Furthermore, in combination with additional fluorescent indicators, the dynamic interplay of ATP, cAMP, and Ca2+ could be visualized in activated brown adipocyte. This set of indicator tools will facilitate future research into energy metabolism.

本文言語English
ページ(範囲)10873-10878
ページ数6
ジャーナルAngewandte Chemie - International Edition
57
34
DOI
出版ステータスPublished - 2018 8 20

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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