Rhythmic expression of circadian clock genes in human leukocytes and beard hair follicle cells

Makiko Watanabe, Akiko Hida, Shingo Kitamura, Minori Enomoto, Yosuke Ohsawa, Yasuko Katayose, Kentaro Nozaki, Yoshiya Moriguchi, Sayaka Aritake, Shigekazu Higuchi, Miyuki Tamura, Mie Kato, Kazuo Mishima

研究成果: Article

27 引用 (Scopus)

抜粋

Evaluating individual circadian rhythm traits is crucial for understanding the human biological clock system. The present study reports characterization of physiological and molecular parameters in 13 healthy male subjects under a constant routine condition, where interfering factors were kept to minimum. We measured hormonal secretion levels and examined temporal expression profiles of circadian clock genes in peripheral leukocytes and beard hair follicle cells. All 13 subjects had prominent daily rhythms in melatonin and cortisol secretion. Significant circadian rhythmicity was found for PER1 in 9 subjects, PER2 in 3 subjects, PER3 in all 13 subjects, and BMAL1 in 8 subjects in leukocytes. Additionally, significant circadian rhythmicity was found for PER1 in 5 of 8 subjects tested, PER2 in 2 subjects, PER3 in 6 subjects, and BMAL1 in 3 subjects in beard hair follicle cells. The phase of PER1 and PER3 rhythms in leukocytes correlated significantly with that of physiological rhythms. Our results demonstrate that leukocytes and beard hair follicle cells possess an endogenous circadian clock and suggest that PER1 and PER3 expression would be appropriate biomarkers and hair follicle cells could be a useful tissue source for the evaluation of biological clock traits in individuals.

元の言語English
ページ(範囲)902-907
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
425
発行部数4
DOI
出版物ステータスPublished - 2012 9 7
外部発表Yes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

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  • これを引用

    Watanabe, M., Hida, A., Kitamura, S., Enomoto, M., Ohsawa, Y., Katayose, Y., Nozaki, K., Moriguchi, Y., Aritake, S., Higuchi, S., Tamura, M., Kato, M., & Mishima, K. (2012). Rhythmic expression of circadian clock genes in human leukocytes and beard hair follicle cells. Biochemical and Biophysical Research Communications, 425(4), 902-907. https://doi.org/10.1016/j.bbrc.2012.08.008