TY - JOUR
T1 - Rhythmic expression of circadian clock genes in human leukocytes and beard hair follicle cells
AU - Watanabe, Makiko
AU - Hida, Akiko
AU - Kitamura, Shingo
AU - Enomoto, Minori
AU - Ohsawa, Yosuke
AU - Katayose, Yasuko
AU - Nozaki, Kentaro
AU - Moriguchi, Yoshiya
AU - Aritake, Sayaka
AU - Higuchi, Shigekazu
AU - Tamura, Miyuki
AU - Kato, Mie
AU - Mishima, Kazuo
PY - 2012/9/7
Y1 - 2012/9/7
N2 - Evaluating individual circadian rhythm traits is crucial for understanding the human biological clock system. The present study reports characterization of physiological and molecular parameters in 13 healthy male subjects under a constant routine condition, where interfering factors were kept to minimum. We measured hormonal secretion levels and examined temporal expression profiles of circadian clock genes in peripheral leukocytes and beard hair follicle cells. All 13 subjects had prominent daily rhythms in melatonin and cortisol secretion. Significant circadian rhythmicity was found for PER1 in 9 subjects, PER2 in 3 subjects, PER3 in all 13 subjects, and BMAL1 in 8 subjects in leukocytes. Additionally, significant circadian rhythmicity was found for PER1 in 5 of 8 subjects tested, PER2 in 2 subjects, PER3 in 6 subjects, and BMAL1 in 3 subjects in beard hair follicle cells. The phase of PER1 and PER3 rhythms in leukocytes correlated significantly with that of physiological rhythms. Our results demonstrate that leukocytes and beard hair follicle cells possess an endogenous circadian clock and suggest that PER1 and PER3 expression would be appropriate biomarkers and hair follicle cells could be a useful tissue source for the evaluation of biological clock traits in individuals.
AB - Evaluating individual circadian rhythm traits is crucial for understanding the human biological clock system. The present study reports characterization of physiological and molecular parameters in 13 healthy male subjects under a constant routine condition, where interfering factors were kept to minimum. We measured hormonal secretion levels and examined temporal expression profiles of circadian clock genes in peripheral leukocytes and beard hair follicle cells. All 13 subjects had prominent daily rhythms in melatonin and cortisol secretion. Significant circadian rhythmicity was found for PER1 in 9 subjects, PER2 in 3 subjects, PER3 in all 13 subjects, and BMAL1 in 8 subjects in leukocytes. Additionally, significant circadian rhythmicity was found for PER1 in 5 of 8 subjects tested, PER2 in 2 subjects, PER3 in 6 subjects, and BMAL1 in 3 subjects in beard hair follicle cells. The phase of PER1 and PER3 rhythms in leukocytes correlated significantly with that of physiological rhythms. Our results demonstrate that leukocytes and beard hair follicle cells possess an endogenous circadian clock and suggest that PER1 and PER3 expression would be appropriate biomarkers and hair follicle cells could be a useful tissue source for the evaluation of biological clock traits in individuals.
KW - Biomarker
KW - Human
KW - PER1
KW - PER3
KW - Surrogate tissue source
UR - http://www.scopus.com/inward/record.url?scp=84865998829&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84865998829&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2012.08.008
DO - 10.1016/j.bbrc.2012.08.008
M3 - Article
C2 - 22902636
AN - SCOPUS:84865998829
VL - 425
SP - 902
EP - 907
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -