Ribosomal Protein S1 Is not Essential for the trans-translation Machinery

Hao Qi, Yoshihiro Shimizu*, Takuya Ueda

*この研究の対応する著者

研究成果: Article査読

24 被引用数 (Scopus)

抄録

In eubacteria, ribosome stalling during protein synthesis is rescued by a tmRNA-derived trans-translation system. Because ribosomal protein S1 specifically binds to tmRNA with high affinity, it is considered to be involved in the trans-translation system. However, the role of S1 in trans-translation is still unclear. To study the function of S1 in the trans-translation system, we constructed an S1-free cell-free translation system. We found that trans-translation proceeded even in the absence of S1. Addition of S1 into the S1-free system did not affect trans-translation efficiency. These results suggest that S1 does not play a role in the trans-translation machinery.

本文言語English
ページ(範囲)845-852
ページ数8
ジャーナルJournal of Molecular Biology
368
3
DOI
出版ステータスPublished - 2007 5月 4
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 分子生物学

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