RNA quality control using external standard RNA

Takema Hasegawa*, Junko Takahashi, Hitoshi Iwahashi

*この研究の対応する著者

研究成果査読

2 被引用数 (Scopus)

抄録

In this paper, we propose a new evaluation method using external standard RNA for quality control of the extracted RNA. RNA Integrity Number and UV absorption are generally used as a basis for RNA quality control; however, these methods do not always reflect the quality of mRNA. While standard RNA is supposedly designed on the basis of mRNA, it has the potential to be used to evaluate the quality of the mRNA. In this study, we took into consideration the three essential factors, viz., yield of mRNA, inhibition to DNA polymerase, and degradation of mRNA for determining the RNA quality using standard RNA. It would be possible to know yield of mRNA and inhibition of the enzyme reaction by adding standard RNA before RNA extraction and looking at standard RNA loss. Degradation was evaluated by comparing the differences in the 3’ and 5’ regions of the RNA. In our study, it was demonstrated that in the crude extract of Saccharomyces cerevisiae, degradation was comparatively higher at the 3’ end of RNA than at the 5’ end. Hence, the degree of RNA degradation can be evaluated by comparing the ratio of degradation from the 3’ and 5’ end.

本文言語English
ページ(範囲)347-353
ページ数7
ジャーナルPolish Journal of Microbiology
67
3
DOI
出版ステータスPublished - 2018
外部発表はい

ASJC Scopus subject areas

  • 微生物学
  • 応用微生物学とバイオテクノロジー
  • 微生物学(医療)

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