Robust processivity of myosin v under off-axis loads

Yusuke Oguchi, Sergey V. Mikhailenko, Takashi Ohki, Adrian O. Olivares, Enrique M. De La Cruz, Shiniichi Ishiwata*

*この研究の対応する著者

研究成果: Article査読

21 被引用数 (Scopus)

抄録

The dimeric motor myosin V transports organelles along actin filament tracks over long distances in cells. Myosin V is a smart 'walker' that is able to swiftly adjust to variable 'road conditions' to continue its processive movement across dense cellular environments. Coordination between the two heads via intramolecular load modulates biochemical kinetics and ensures highly efficient unidirectional motion. However, little is known about how load-induced regulation of the processive stepping occurs in vivo, where myosin V experiences significant off-axis loads applied in various directions. To reveal how myosin V remains processive in cells, we measured the effect of the off-axis loads, applied to individual actomyosin V bonds in a range of angles, on the coordination between the two heads and myosin V processive stepping. We found that myosin V remains highly processive under diagonal loads owing to asymmetrical ADP affinities and that the native 6IQ lever optimizes the subunit coordination, which indicates that myosin V is designed to be an intracellular transporter.

本文言語English
ページ(範囲)300-305
ページ数6
ジャーナルNature Chemical Biology
6
4
DOI
出版ステータスPublished - 2010 4

ASJC Scopus subject areas

  • 分子生物学
  • 細胞生物学

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