Robustness of predictions of extremely thermally stable proteins in ancient organisms

Satoshi Akanuma, Shin ichi Yokobori, Yoshiki Nakajima, Mizumo Bessho, Akihiko Yamagishi

    研究成果: Article

    15 引用 (Scopus)

    抄録

    A number of studies have addressed the environmental temperatures experienced by ancient life. Computational studies using a nonhomogeneous evolution model have estimated ancestral G + C contents of ribosomal RNAs and the amino acid compositions of ancestral proteins, generating hypotheses regarding the mesophilic last universal common ancestor. In contrast, our previous study computationally reconstructed ancestral amino acid sequences of nucleoside diphosphate kinases using a homogeneous model and then empirically resurrected the ancestral proteins. The thermal stabilities of these ancestral proteins were equivalent to or greater than those of extant homologous thermophilic proteins, supporting the thermophilic universal ancestor theory. In this study, we reinferred ancestral sequences using a dataset from which hyperthermophilic sequences were excluded. We also reinferred ancestral sequences using a nonhomogeneous evolution model. The newly reconstructed ancestral proteins are still thermally stable, further supporting the hypothesis that the ancient organisms contained thermally stable proteins and therefore that they were thermophilic.

    元の言語English
    ページ(範囲)2954-2962
    ページ数9
    ジャーナルEvolution
    69
    発行部数11
    DOI
    出版物ステータスPublished - 2015 11 1

    Fingerprint

    protein
    prediction
    organisms
    Proteins
    proteins
    Nucleoside-Diphosphate Kinase
    ancestry
    amino acid
    nucleoside-diphosphate kinase
    Ribosomal RNA
    Protein Stability
    Base Composition
    common ancestry
    Amino Acid Sequence
    thermal stability
    amino acid composition
    Hot Temperature
    organism
    RNA
    ambient temperature

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Ecology, Evolution, Behavior and Systematics
    • Genetics

    これを引用

    Akanuma, S., Yokobori, S. I., Nakajima, Y., Bessho, M., & Yamagishi, A. (2015). Robustness of predictions of extremely thermally stable proteins in ancient organisms. Evolution, 69(11), 2954-2962. https://doi.org/10.1111/evo.12779

    Robustness of predictions of extremely thermally stable proteins in ancient organisms. / Akanuma, Satoshi; Yokobori, Shin ichi; Nakajima, Yoshiki; Bessho, Mizumo; Yamagishi, Akihiko.

    :: Evolution, 巻 69, 番号 11, 01.11.2015, p. 2954-2962.

    研究成果: Article

    Akanuma, S, Yokobori, SI, Nakajima, Y, Bessho, M & Yamagishi, A 2015, 'Robustness of predictions of extremely thermally stable proteins in ancient organisms', Evolution, 巻. 69, 番号 11, pp. 2954-2962. https://doi.org/10.1111/evo.12779
    Akanuma, Satoshi ; Yokobori, Shin ichi ; Nakajima, Yoshiki ; Bessho, Mizumo ; Yamagishi, Akihiko. / Robustness of predictions of extremely thermally stable proteins in ancient organisms. :: Evolution. 2015 ; 巻 69, 番号 11. pp. 2954-2962.
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