抄録
A number of studies have addressed the environmental temperatures experienced by ancient life. Computational studies using a nonhomogeneous evolution model have estimated ancestral G + C contents of ribosomal RNAs and the amino acid compositions of ancestral proteins, generating hypotheses regarding the mesophilic last universal common ancestor. In contrast, our previous study computationally reconstructed ancestral amino acid sequences of nucleoside diphosphate kinases using a homogeneous model and then empirically resurrected the ancestral proteins. The thermal stabilities of these ancestral proteins were equivalent to or greater than those of extant homologous thermophilic proteins, supporting the thermophilic universal ancestor theory. In this study, we reinferred ancestral sequences using a dataset from which hyperthermophilic sequences were excluded. We also reinferred ancestral sequences using a nonhomogeneous evolution model. The newly reconstructed ancestral proteins are still thermally stable, further supporting the hypothesis that the ancient organisms contained thermally stable proteins and therefore that they were thermophilic.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 2954-2962 |
| ページ数 | 9 |
| ジャーナル | Evolution |
| 巻 | 69 |
| 発行部数 | 11 |
| DOI | |
| 出版物ステータス | Published - 2015 11 1 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Ecology, Evolution, Behavior and Systematics
- Genetics
これを引用
Robustness of predictions of extremely thermally stable proteins in ancient organisms. / Akanuma, Satoshi; Yokobori, Shin ichi; Nakajima, Yoshiki; Bessho, Mizumo; Yamagishi, Akihiko.
：: Evolution, 巻 69, 番号 11, 01.11.2015, p. 2954-2962.研究成果: Article
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TY - JOUR
T1 - Robustness of predictions of extremely thermally stable proteins in ancient organisms
AU - Akanuma, Satoshi
AU - Yokobori, Shin ichi
AU - Nakajima, Yoshiki
AU - Bessho, Mizumo
AU - Yamagishi, Akihiko
PY - 2015/11/1
Y1 - 2015/11/1
N2 - A number of studies have addressed the environmental temperatures experienced by ancient life. Computational studies using a nonhomogeneous evolution model have estimated ancestral G + C contents of ribosomal RNAs and the amino acid compositions of ancestral proteins, generating hypotheses regarding the mesophilic last universal common ancestor. In contrast, our previous study computationally reconstructed ancestral amino acid sequences of nucleoside diphosphate kinases using a homogeneous model and then empirically resurrected the ancestral proteins. The thermal stabilities of these ancestral proteins were equivalent to or greater than those of extant homologous thermophilic proteins, supporting the thermophilic universal ancestor theory. In this study, we reinferred ancestral sequences using a dataset from which hyperthermophilic sequences were excluded. We also reinferred ancestral sequences using a nonhomogeneous evolution model. The newly reconstructed ancestral proteins are still thermally stable, further supporting the hypothesis that the ancient organisms contained thermally stable proteins and therefore that they were thermophilic.
AB - A number of studies have addressed the environmental temperatures experienced by ancient life. Computational studies using a nonhomogeneous evolution model have estimated ancestral G + C contents of ribosomal RNAs and the amino acid compositions of ancestral proteins, generating hypotheses regarding the mesophilic last universal common ancestor. In contrast, our previous study computationally reconstructed ancestral amino acid sequences of nucleoside diphosphate kinases using a homogeneous model and then empirically resurrected the ancestral proteins. The thermal stabilities of these ancestral proteins were equivalent to or greater than those of extant homologous thermophilic proteins, supporting the thermophilic universal ancestor theory. In this study, we reinferred ancestral sequences using a dataset from which hyperthermophilic sequences were excluded. We also reinferred ancestral sequences using a nonhomogeneous evolution model. The newly reconstructed ancestral proteins are still thermally stable, further supporting the hypothesis that the ancient organisms contained thermally stable proteins and therefore that they were thermophilic.
KW - Ancestral sequence reconstruction
KW - Nucleoside diphosphate kinase
KW - Phylogenetic tree
KW - Substitution model
KW - Unfolding temperature
UR - http://www.scopus.com/inward/record.url?scp=84955214204&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84955214204&partnerID=8YFLogxK
U2 - 10.1111/evo.12779
DO - 10.1111/evo.12779
M3 - Article
C2 - 26404857
AN - SCOPUS:84955214204
VL - 69
SP - 2954
EP - 2962
JO - Evolution; international journal of organic evolution
JF - Evolution; international journal of organic evolution
SN - 0014-3820
IS - 11
ER -