Robustness of predictions of extremely thermally stable proteins in ancient organisms

Satoshi Akanuma, Shin ichi Yokobori, Yoshiki Nakajima, Mizumo Bessho, Akihiko Yamagishi*

*この研究の対応する著者

    研究成果: Article査読

    21 被引用数 (Scopus)

    抄録

    A number of studies have addressed the environmental temperatures experienced by ancient life. Computational studies using a nonhomogeneous evolution model have estimated ancestral G + C contents of ribosomal RNAs and the amino acid compositions of ancestral proteins, generating hypotheses regarding the mesophilic last universal common ancestor. In contrast, our previous study computationally reconstructed ancestral amino acid sequences of nucleoside diphosphate kinases using a homogeneous model and then empirically resurrected the ancestral proteins. The thermal stabilities of these ancestral proteins were equivalent to or greater than those of extant homologous thermophilic proteins, supporting the thermophilic universal ancestor theory. In this study, we reinferred ancestral sequences using a dataset from which hyperthermophilic sequences were excluded. We also reinferred ancestral sequences using a nonhomogeneous evolution model. The newly reconstructed ancestral proteins are still thermally stable, further supporting the hypothesis that the ancient organisms contained thermally stable proteins and therefore that they were thermophilic.

    本文言語English
    ページ(範囲)2954-2962
    ページ数9
    ジャーナルEvolution
    69
    11
    DOI
    出版ステータスPublished - 2015 11 1

    ASJC Scopus subject areas

    • 農業および生物科学(全般)
    • 生態、進化、行動および分類学
    • 遺伝学

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