Role of myosin II tail sequences in its function and localization at the cleavage furrow in Dictyostelium

Shi Shu, Randall J. Lee, Janine M. LeBlanc-Straceski, Taro Q.P. Uyeda*

*この研究の対応する著者

研究成果: Article査読

19 被引用数 (Scopus)

抄録

Cytoplasmic myosin II accumulates in the cleavage furrow and provides the force for cytokinesis in animal and amoeboid cells. One model proposes that a specific domain in the myosin II tail is responsible for its localization, possibly by interacting with a factor concentrated in the equatorial region. To test this possibility, we have expressed myosins carrying mutations in the tail domain in a strain of Dictyostelium cells from which the endogenous myosin heavy chain gene has been deleted. The mutations used in this study include four internal tail deletions: MyΔ824-941, MyΔ943-1464, MyΔ943-1194 and MyΔ1156-1464. Contrary to the prediction of the hypothesis, immunofluorescence staining demonstrated that all mutant myosins were able to move toward the furrow region. Chimeric myosins, which consisted of a Dictyostelium myosin head and chicken skeletal myosin tail, also efficiently localized to the cleavage furrow. All these deletion and chimeric mutant myosins, except for MyΔ943-1464, the largest deletion mutant, were able to support cytokinesis in suspension. Our data suggest that there is no single specific domain in the tail of Dictyostelium myosin II that is required for its functioning at and localization to the cleavage furrow.

本文言語English
ページ(範囲)2195-2201
ページ数7
ジャーナルJournal of Cell Science
112
13
出版ステータスPublished - 1999
外部発表はい

ASJC Scopus subject areas

  • 細胞生物学

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