Role of timer and sizer in regulation of Chlamydomonas cell cycle

Kazunori Matsumura, Toshiki Yagi, Kenji Yasuda

研究成果: Article査読

36 被引用数 (Scopus)

抄録

To estimate the role that time and size had in controlling the Chlamydomonas cell cycle, we used a new on-chip single-cell microcultivation system, which involved the direct observation of single cells captured in microchambers made on a thin glass slide. The dependence of the pattern of energy supply for cells on its cell cycle was examined through a series of different intensities of continuous illumination in a minimal medium, and we found that cell division occurred when cells reached the critical size, which was 2.2 times larger than that of the newly created cells. When illumination stopped before cells reached the critical size, even though growth had stopped, they continued dividing during the delay time, which was shorter when cells were larger. With re-illumination after darkness, cells began to grow again and the timing of cell division was again controlled by the critical size. This indicates that the co-existence of two cell cycle regulation mechanisms and the sizer mechanism had a stronger influence than the timer.

本文言語English
ページ(範囲)1042-1049
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
306
4
DOI
出版ステータスPublished - 2003 7 11
外部発表はい

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

フィンガープリント 「Role of timer and sizer in regulation of Chlamydomonas cell cycle」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル