Role of timer and sizer in regulation of Chlamydomonas cell cycle

Kazunori Matsumura, Toshiki Yagi, Kenji Yasuda*

*この研究の対応する著者

研究成果: Article査読

37 被引用数 (Scopus)

抄録

To estimate the role that time and size had in controlling the Chlamydomonas cell cycle, we used a new on-chip single-cell microcultivation system, which involved the direct observation of single cells captured in microchambers made on a thin glass slide. The dependence of the pattern of energy supply for cells on its cell cycle was examined through a series of different intensities of continuous illumination in a minimal medium, and we found that cell division occurred when cells reached the critical size, which was 2.2 times larger than that of the newly created cells. When illumination stopped before cells reached the critical size, even though growth had stopped, they continued dividing during the delay time, which was shorter when cells were larger. With re-illumination after darkness, cells began to grow again and the timing of cell division was again controlled by the critical size. This indicates that the co-existence of two cell cycle regulation mechanisms and the sizer mechanism had a stronger influence than the timer.

本文言語English
ページ(範囲)1042-1049
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
306
4
DOI
出版ステータスPublished - 2003 7月 11
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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